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<p><i>Gpi7</i> gene, encoding the catalytic subunit of GPI ethanolamine-phosphate (Etn-P) transferase II, is primarily involved in the synthesis, maturation, and sorting of GPI-anchored proteins (GPI-APs), thereby playing a crucial part in cell wall functions and host-pathogen interactions. This study aimed to investigate the role of <i>Gpi7</i> (here designated <i>CcGpi7</i>) in <i>Corynespora cassiicola</i>, a devastating fungal pathogen causing leaf spot in cucumber and many other cash crops. We systematically identified and inventoried 138 GPI-APs in <i>C. cassiicola</i>, followed by a detailed structural characterization of the CcGpi7 protein that is strongly induced during infection. Homologous recombination was employed to construct a <i>CcGpi7</i>-deleted mutant (Δ<i>CcGpi7</i>) and its corresponding complementary strain (c<i>CcGpi7</i>). Compared with the wild type and c<i>CcGpi7</i>, deletion of <i>CcGpi7</i> led to markedly reduced vegetative growth and conidia formation. The Δ<i>CcGpi7</i> mutant displayed obvious defects in cell wall architecture, manifested as enhanced susceptibility to cell wall-perturbing agents and degrading enzymes. Under stress conditions, Δ<i>CcGpi7</i> exhibited increased sensitivity to KCl but reduced sensitivity to sorbitol and H<sub>2</sub>O<sub>2</sub>. Pathogenicity assays revealed a dramatic attenuation in the virulence of Δ<i>CcGpi7</i> on cucumber leaves, directly correlating with its impaired ability to form invasive hyphae. Transcriptome profiling identified a total of 3,604 differentially expressed genes in Δ<i>CcGpi7</i>, which were enriched in multiple processes including cellular growth and development, cell wall organization, sporulation, and unexpectedly, transcription and translation. Together, our findings demonstrate that CcGpi7 exerts pleotropic effects on vegetative growth, reproduction, cell wall integrity, and pathogenesis of <i>C. cassiicola</i>. This work lays a theoretical foundation for developing CcGpi7-targeted control strategies against cucumber target spot disease.</p>

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Multifaceted roles of CcGpi7, a phosphoethanolamine transferase, in physiology and virulence of Corynespora cassiicola

  • 期刊论文

  • 发表日期：

    2026年5月11日

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  • Chao Xu,
  • Mengyuan Hou,
  • Qingzhou Ma,
  • Yue-hua Geng,
  • Rui Zang,
  • Taibo Liang,
  • Yashuang Guo,
  • Mengmeng Yang,
  • Meng Zhang

摘要

Gpi7 gene, encoding the catalytic subunit of GPI ethanolamine-phosphate (Etn-P) transferase II, is primarily involved in the synthesis, maturation, and sorting of GPI-anchored proteins (GPI-APs), thereby playing a crucial part in cell wall functions and host-pathogen interactions. This study aimed to investigate the role of Gpi7 (here designated CcGpi7) in Corynespora cassiicola, a devastating fungal pathogen causing leaf spot in cucumber and many other cash crops. We systematically identified and inventoried 138 GPI-APs in C. cassiicola, followed by a detailed structural characterization of the CcGpi7 protein that is strongly induced during infection. Homologous recombination was employed to construct a CcGpi7-deleted mutant (ΔCcGpi7) and its corresponding complementary strain (cCcGpi7). Compared with the wild type and cCcGpi7, deletion of CcGpi7 led to markedly reduced vegetative growth and conidia formation. The ΔCcGpi7 mutant displayed obvious defects in cell wall architecture, manifested as enhanced susceptibility to cell wall-perturbing agents and degrading enzymes. Under stress conditions, ΔCcGpi7 exhibited increased sensitivity to KCl but reduced sensitivity to sorbitol and H2O2. Pathogenicity assays revealed a dramatic attenuation in the virulence of ΔCcGpi7 on cucumber leaves, directly correlating with its impaired ability to form invasive hyphae. Transcriptome profiling identified a total of 3,604 differentially expressed genes in ΔCcGpi7, which were enriched in multiple processes including cellular growth and development, cell wall organization, sporulation, and unexpectedly, transcription and translation. Together, our findings demonstrate that CcGpi7 exerts pleotropic effects on vegetative growth, reproduction, cell wall integrity, and pathogenesis of C. cassiicola. This work lays a theoretical foundation for developing CcGpi7-targeted control strategies against cucumber target spot disease.