Sustainable biotreatment of vegetable oil extraction wastewater by Rhodotorula glutinis with evaluation of its potential for biodiesel and feed applications
摘要
Oily wastewater containing high concentrations of organic and fatty compounds requires effective treatment before environmental discharge. In this study, the oleaginous and lipid-degrading yeast Rhodotorula glutinis was employed for biotreatment of wastewater from an oil extraction factory. The process achieved 64.1% and 65.7% reduction in biochemical oxygen demand (BOD) and chemical oxygen demand (COD) levels, respectively, while sulfate removal reached 99.7% under controlled laboratory conditions using sterilized wastewater. Optimization experiments revealed that the highest biomass concentration was obtained at 25 °C and 70–80% wastewater concentration. Amino acid and fatty acid analysis of the biomass harvested after biotreatment confirmed a significant content of essential amino acids and omega‑6 fatty acids, indicating its potential as a valuable supplement for poultry feed. Evaluation of the biodiesel properties predicted from the physicochemical parameters of the biomass lipids indicated that the cetane number, density, and kinematic viscosity comply with biodiesel ASTM D6751 standard specifications. Cytotoxicity assessment of the harvested biomass from biotreatment on HEK 293 cell line determined the IC50 value of 237.9 µg/mL.