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<p>To explore the inhibitory effect of 1% ascorbic acid on spoilage bacteria under super-chilled storage, tilapia fillets either with natural microbiota or inoculated with <i>Pseudomonas bubulae</i> P1, a dominant spoiler derived from tilapia, were employed in this research. Results showed that ascorbic acid significantly inhibited bacterial growth, delayed sensory spoilage, and reduced the levels of total volatile basic nitrogen (TVB-N) in both types of fillets stored at -1.5&#xa0;°C. Further analysis indicated that the reduction in TVB-N was attributed to the diminished ammonia production by P1. Transcriptomic analysis of P1 revealed that exposure to ascorbic acid significantly up-regulates the expression of 14 genes associated with oxidative stress response. Some genes related to ribosome biosynthesis and energy production were also up-regulated. These findings suggest that P1 experiences oxidative stress when exposed to ascorbic acid. Besides, the genes <i>arcC</i> and <i>glnA</i> responsible for ammonia assimilation are significantly up-regulated, which explains the reduced production of ammonia.</p>

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Inhibition of ascorbic acid on Pseudomonas bubulae under super-chilled storage

  • 期刊论文

  • 发表日期：

    2026年4月10日

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  • Anjie Liang,
  • Huiping Chen,
  • Yihong Duan,
  • Xiaolan Miao,
  • Jianyin Miao,
  • Shan Duan

摘要

To explore the inhibitory effect of 1% ascorbic acid on spoilage bacteria under super-chilled storage, tilapia fillets either with natural microbiota or inoculated with Pseudomonas bubulae P1, a dominant spoiler derived from tilapia, were employed in this research. Results showed that ascorbic acid significantly inhibited bacterial growth, delayed sensory spoilage, and reduced the levels of total volatile basic nitrogen (TVB-N) in both types of fillets stored at -1.5 °C. Further analysis indicated that the reduction in TVB-N was attributed to the diminished ammonia production by P1. Transcriptomic analysis of P1 revealed that exposure to ascorbic acid significantly up-regulates the expression of 14 genes associated with oxidative stress response. Some genes related to ribosome biosynthesis and energy production were also up-regulated. These findings suggest that P1 experiences oxidative stress when exposed to ascorbic acid. Besides, the genes arcC and glnA responsible for ammonia assimilation are significantly up-regulated, which explains the reduced production of ammonia.