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<p>Huanglongbing (HLB, <i>‘Candidatus</i> Liberibacter asiaticus’) is one of the most devastating pathogens in citrus domain. Here, we present the nearly complete genome sequence of a CR-NGP1 strain obtained a from symptomatic Nagpur Mandarin (<i>Citrus reticulata</i>) tree in the Nagpur region of Central India. High-throughput sequencing on the Illumina NovaSeq 6000 platform generated ~ 85.7&#xa0;million paired-end reads, 63.5&#xa0;million paired-end reads and 14.8&#xa0;million paired-end reads for sample <i>C</i>Las_001, <i>C</i>Las_002 and <i>C</i>Las_003 each with 150&#xa0;bp read length, respectively. Two assembly strategies were used: (i) reference-based assembly with SPAdes produced a draft genome of ~ 1.19&#xa0;Mb with assembly comprised 149 contigs, with an N50 of 14,173&#xa0;bp, longest contig of 39,711&#xa0;bp, and an overall GC content of 36.27%. (ii) KBase CONCOCT binning v1.1 applied to all 3 samples produced a nearly complete CR-NGP1 genome of ~ 1,156,009 bases with assembly of 93 contig, with an N50 of 17,668&#xa0;bp, a longest contig of 39,711&#xa0;bp, and an overall GC content of 36.4%. This resource of a <i>C</i>Las genome from Central India provides important insights to understand genetic diversity of <i>C</i>Las strains and will facilitate comparative genomics and epidemiological studies of Huanglongbing.</p>

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Draft genome sequence CR-NGP1 strain of ‘Candidatus Liberibacter asiaticus’ (CLas) from the host Citrus reticulata (Nagpur mandarin) from Central India

  • 期刊论文

  • 发表日期：

    2026年5月8日

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  • Rushikesh D. Bharsakale,
  • Mrugendra G. Gubyad,
  • Prasanth Tej Kumar Jagannadham,
  • Sunil B. Kokane,
  • Ashish J. Warghane,
  • Amol D. Kokane,
  • Dilip Kumar Ghosh

摘要

Huanglongbing (HLB, ‘Candidatus Liberibacter asiaticus’) is one of the most devastating pathogens in citrus domain. Here, we present the nearly complete genome sequence of a CR-NGP1 strain obtained a from symptomatic Nagpur Mandarin (Citrus reticulata) tree in the Nagpur region of Central India. High-throughput sequencing on the Illumina NovaSeq 6000 platform generated ~ 85.7 million paired-end reads, 63.5 million paired-end reads and 14.8 million paired-end reads for sample CLas_001, CLas_002 and CLas_003 each with 150 bp read length, respectively. Two assembly strategies were used: (i) reference-based assembly with SPAdes produced a draft genome of ~ 1.19 Mb with assembly comprised 149 contigs, with an N50 of 14,173 bp, longest contig of 39,711 bp, and an overall GC content of 36.27%. (ii) KBase CONCOCT binning v1.1 applied to all 3 samples produced a nearly complete CR-NGP1 genome of ~ 1,156,009 bases with assembly of 93 contig, with an N50 of 17,668 bp, a longest contig of 39,711 bp, and an overall GC content of 36.4%. This resource of a CLas genome from Central India provides important insights to understand genetic diversity of CLas strains and will facilitate comparative genomics and epidemiological studies of Huanglongbing.