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<p><i>Lactobacillus gallinarum</i> Y86, isolated from broiler ileal mucosa under strict anaerobiosis (85% N₂/10% CO₂/5% H₂), demonstrates significant potential as a microbial feed additive for antibiotic-free farming. 16&#xa0;S rRNA sequencing (99.79% identity to <i>L. gallinarum</i> ATCC 33199; GenBank ON248243) confirmed its taxonomy. Stationary-phase cultures secreted a heat-stable antimicrobial that produced inhibition zones of 21.53 ± 0.34&#xa0;mm against <i>Salmonella enterica serovar pullorum</i> and 11.90 ± 0.52&#xa0;mm against <i>Staphylococcus aureus</i>, retaining 87.30% activity after 120&#xa0;°C for 15&#xa0;min; sensitivity to trypsin and lipase indicates a proteolipid nature. Y86 endured pH 2.0 for 3&#xa0;h (63.37% survival) before programmed lysis at 4&#xa0;h, then recovered to 92.44% viability under intestinal conditions and maintained 45.36% viability in 0.5% bile. High surface hydrophobicity (85.71%) drove auto-aggregation to 97.99% within 24&#xa0;h, supporting strong epithelial adhesion. The strain was susceptible to β-lactams, macrolides, and vancomycin, intrinsically resistant to tetracyclines and quinolones, and non-haemolytic, meeting EFSA-QPS safety criteria. Collectively, its thermostable antimicrobial production, timed gastric lysis, intestinal resilience, and proven safety identify Y86 as an industrially compatible candidate for antibiotic-free poultry feeds, advancing microbiota-based alternatives to growth-promoting antibiotics.</p>

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Characterization of L. gallinarum Y86: heat-stable antimicrobials and gastrointestinal adaptation

  • 期刊论文

  • 发表日期：

    2025年12月31日

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  • Jia-Bao Zhang,
  • Yong-Jun Yang,
  • Zhen-Zhen Liu

摘要

Lactobacillus gallinarum Y86, isolated from broiler ileal mucosa under strict anaerobiosis (85% N₂/10% CO₂/5% H₂), demonstrates significant potential as a microbial feed additive for antibiotic-free farming. 16 S rRNA sequencing (99.79% identity to L. gallinarum ATCC 33199; GenBank ON248243) confirmed its taxonomy. Stationary-phase cultures secreted a heat-stable antimicrobial that produced inhibition zones of 21.53 ± 0.34 mm against Salmonella enterica serovar pullorum and 11.90 ± 0.52 mm against Staphylococcus aureus, retaining 87.30% activity after 120 °C for 15 min; sensitivity to trypsin and lipase indicates a proteolipid nature. Y86 endured pH 2.0 for 3 h (63.37% survival) before programmed lysis at 4 h, then recovered to 92.44% viability under intestinal conditions and maintained 45.36% viability in 0.5% bile. High surface hydrophobicity (85.71%) drove auto-aggregation to 97.99% within 24 h, supporting strong epithelial adhesion. The strain was susceptible to β-lactams, macrolides, and vancomycin, intrinsically resistant to tetracyclines and quinolones, and non-haemolytic, meeting EFSA-QPS safety criteria. Collectively, its thermostable antimicrobial production, timed gastric lysis, intestinal resilience, and proven safety identify Y86 as an industrially compatible candidate for antibiotic-free poultry feeds, advancing microbiota-based alternatives to growth-promoting antibiotics.