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<p><i>Staphylococcus aureus</i>, a gram-positive bacterium, is the prevalent cause of numerous infections. Its ability to form biofilms significantly enhances its pathogenicity, resulting in increased antibiotic resistance and evasion of the host immune response. Poly-β-(1,6)-N-acetylglucosamine (PNAG) plays a crucial role in the formation and maintenance of <i>S. aureus</i> biofilms. In this study, we heterologously expressed <i>Ka</i>PgaB from <i>Klebsiella aerogenes</i> and evaluated its efficacy in both degrading and inhibiting <i>S. aureus</i> biofilm formation. Additionally, we investigated the combined effects of <i>Ka</i>PgaB with DNase I and papain. Our results demonstrated that <i>Ka</i>PgaB alone removed up to 81% of biofilm biomass within 4&#xa0;h when used at a concentration of 0.5&#xa0;mg/mL. Moreover, when the enzyme was applied sequentially with DNase I, approximately 97% of adhered biofilms were removed. We also observed significant inhibition of biofilm formation across <i>S. aureus</i> strains. The findings presented in this study might be useful for the development of enzymatic tools capable of degrading <i>S. aureus</i> PNAG-based biofilms.</p>

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Klebsiella aerogenes PgaB orthologue can efficiently hydrolyze Staphylococcus aureus biofilms

  • 期刊论文

  • 发表日期：

    2025年10月3日

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  • Jéssica Pinheiro Silva,
  • Andrei Nicoli Gebieluca Dabul,
  • Vera Lúcia Mores Rall,
  • Caroline Rosa Silva,
  • Luís Antônio Esmerino,
  • Marcos Pileggi,
  • Felipe Francisco Tuon,
  • Mario de Oliveira Neto,
  • Darlan Nakayama,
  • Alejandra Estela Miranda,
  • Pedro Ricardo Vieira Hamann,
  • Igor Polikarpov

摘要

Staphylococcus aureus, a gram-positive bacterium, is the prevalent cause of numerous infections. Its ability to form biofilms significantly enhances its pathogenicity, resulting in increased antibiotic resistance and evasion of the host immune response. Poly-β-(1,6)-N-acetylglucosamine (PNAG) plays a crucial role in the formation and maintenance of S. aureus biofilms. In this study, we heterologously expressed KaPgaB from Klebsiella aerogenes and evaluated its efficacy in both degrading and inhibiting S. aureus biofilm formation. Additionally, we investigated the combined effects of KaPgaB with DNase I and papain. Our results demonstrated that KaPgaB alone removed up to 81% of biofilm biomass within 4 h when used at a concentration of 0.5 mg/mL. Moreover, when the enzyme was applied sequentially with DNase I, approximately 97% of adhered biofilms were removed. We also observed significant inhibition of biofilm formation across S. aureus strains. The findings presented in this study might be useful for the development of enzymatic tools capable of degrading S. aureus PNAG-based biofilms.