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<p>Microbial methanation, which produces CH<sub>4</sub> from H<sub>2</sub> and CO<sub>2</sub> (H<sub>2</sub>/CO<sub>2</sub>) using hydrogenotrophic methanogens, converts CO<sub>2</sub> into a resource. The proposed system utilizes an isolated strain of hydrogenotrophic methanogen and an anaerobic microbial community from a deep aquifer where H<sub>2</sub> and CO<sub>2</sub> are produced via biodegradation of organic matter by fermentative bacteria, which CH<sub>4</sub> is then produced from H<sub>2</sub> and CO<sub>2</sub> by hydrogenotrophic methanogens. The strain and microbial community were added separately to liquid medium in serum bottles. An H<sub>2</sub>/CO<sub>2</sub> mixture was added to each bottle’s gas phase and incubated. CH<sub>4</sub> was produced continuously across both cultures. However, CH<sub>4</sub> production rates in the bottle with a single methanogen strain gradually decreased during cultivation with H<sub>2</sub> and CO<sub>2</sub> exchange. In contrast, the culture using the microbial community derived from anaerobic groundwater maintained consistent CH<sub>4</sub> production throughout the experiment. Analysis of organic acids in the media revealed that the accumulation of formate, acetate, and propionate in the microbial community culture was significantly lower than in the single-strain methanogen culture. These findings suggest that anaerobic bacteria efficiently decompose and consume organic acids that inhibit methanogenic activity, improving the culture environment. This study demonstrates the microbial community’s effectiveness in supporting and sustaining a microbial methanation system.</p>

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Role of organic acid-degrading bacteria in a microbial methanation system using a subterranean microbial community

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月28日

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  • Makoto Kawano,
  • Kohei Ikeura,
  • Hiroki Taniguchi,
  • Minako Terao,
  • Kota Ichikawa,
  • Takumi Higashi,
  • Hiroyuki Futamata,
  • Hiroyuki Kimura

摘要

Microbial methanation, which produces CH4 from H2 and CO2 (H2/CO2) using hydrogenotrophic methanogens, converts CO2 into a resource. The proposed system utilizes an isolated strain of hydrogenotrophic methanogen and an anaerobic microbial community from a deep aquifer where H2 and CO2 are produced via biodegradation of organic matter by fermentative bacteria, which CH4 is then produced from H2 and CO2 by hydrogenotrophic methanogens. The strain and microbial community were added separately to liquid medium in serum bottles. An H2/CO2 mixture was added to each bottle’s gas phase and incubated. CH4 was produced continuously across both cultures. However, CH4 production rates in the bottle with a single methanogen strain gradually decreased during cultivation with H2 and CO2 exchange. In contrast, the culture using the microbial community derived from anaerobic groundwater maintained consistent CH4 production throughout the experiment. Analysis of organic acids in the media revealed that the accumulation of formate, acetate, and propionate in the microbial community culture was significantly lower than in the single-strain methanogen culture. These findings suggest that anaerobic bacteria efficiently decompose and consume organic acids that inhibit methanogenic activity, improving the culture environment. This study demonstrates the microbial community’s effectiveness in supporting and sustaining a microbial methanation system.