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<p>The microbial reduction of hexavalent chromium (Cr(VI)), particularly by bacteria, has been extensively studied, revealing varying removal mechanisms among different strains. This investigation identified a novel bacterial strain, <i>Staphylococcus succinus</i> subsp<i>. Succinus</i> AMG-D1, which was isolated from mining soil and Cr(VI)-resistant. This strain demonstrated the capability to completely remove a high concentration of 200&#xa0;mg/L Cr(VI) within 120&#xa0;h at pH 7 and 35&#xa0;°C. Moreover, it effectively reduced repeated contamination of 100&#xa0;mg/L Cr(VI). Scanning electron microscopy analysis confirmed the strain's high resistance to Cr(VI), coupled with the secretion of a viscous material, possibly metal-adsorbing exopolysaccharides. Energy dispersive X-ray peaks analysis revealed characteristic chromium peaks, indicating the presence of adsorbed Cr(VI) and/or precipitated species of reduced chromium (Cr(III)). Further investigations into Cr(VI) removal mechanisms have revealed a detoxification process involving adsorption, accumulation, and enzymatically mediated biological reduction. This reduction predominantly occurs through constitutive cytoplasmic proteins. Moreover, a protein with a molecular weight of approximately 30&#xa0;kDa was identified on an SDS-PAGE gel, potentially responsible for Cr(VI) reduction. The remarkable resistance and substantial reduction capabilities of <i>Staphylococcus succinus</i> position it as a promising strain for bioremediation applications.</p>

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Bioreduction of hexavalent chromium and removal mechanisms using Staphylococcus succinus

  • 期刊论文

  • 发表日期：

    2025年4月28日

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  • Kaoutar Harboul,
  • Houda Chtibi,
  • Halima Amakdouf,
  • Khalil Hammani,
  • Abdenbi El-Karkouri

摘要

The microbial reduction of hexavalent chromium (Cr(VI)), particularly by bacteria, has been extensively studied, revealing varying removal mechanisms among different strains. This investigation identified a novel bacterial strain, Staphylococcus succinus subsp. Succinus AMG-D1, which was isolated from mining soil and Cr(VI)-resistant. This strain demonstrated the capability to completely remove a high concentration of 200 mg/L Cr(VI) within 120 h at pH 7 and 35 °C. Moreover, it effectively reduced repeated contamination of 100 mg/L Cr(VI). Scanning electron microscopy analysis confirmed the strain's high resistance to Cr(VI), coupled with the secretion of a viscous material, possibly metal-adsorbing exopolysaccharides. Energy dispersive X-ray peaks analysis revealed characteristic chromium peaks, indicating the presence of adsorbed Cr(VI) and/or precipitated species of reduced chromium (Cr(III)). Further investigations into Cr(VI) removal mechanisms have revealed a detoxification process involving adsorption, accumulation, and enzymatically mediated biological reduction. This reduction predominantly occurs through constitutive cytoplasmic proteins. Moreover, a protein with a molecular weight of approximately 30 kDa was identified on an SDS-PAGE gel, potentially responsible for Cr(VI) reduction. The remarkable resistance and substantial reduction capabilities of Staphylococcus succinus position it as a promising strain for bioremediation applications.