Scholarlink

<p>Enantiopure (<i>S</i>)-2-methylbutanoic acid [(<i>S</i>)-2-MBA] is a high-value chiral compound with applications in fragrances, pharmaceuticals, and agrochemicals. However, conventional chemical synthesis lacks stereoselectivity, while existing biosynthetic methods suffer from low yield and purity. Here, we report a novel microbial process using <i>Bacillus spizizenii</i> ATCC 6633 for efficient (<i>S</i>)-2-MBA production via L-isoleucine catabolism. Through targeted screening of rhizospheric soil isolates and <i>Bacillaceae</i> strains, ATCC 6633 demonstrated superior performance, producing 3.67&#xa0;g/L (<i>S</i>)-2-MBA with 99.32% enantiomeric excess (<i>ee</i>) under optimized conditions (45&#xa0;°C, 8% inoculation, 5&#xa0;g/L glucose, and 8&#xa0;g/L L-isoleucine). A 58.92% conversion efficiency was achieved, and a simplified purification process recovered 63.90% product with 97.32% purity. Mechanistic studies suggested glucose depletion triggered (<i>S</i>)-2-MBA accumulation, aligning with starvation-induced secondary metabolism. This cost-effective, eco-friendly approach eliminates racemic separation steps and harsh reagents, positioning ATCC 6633 as a promising biocatalyst for sustainable (<i>S</i>)-2-MBA production.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Microbial synthesis of enantiopure (S)-2-methylbutanoic acid via L-isoleucine catabolism in Bacillus spizizenii

  • 期刊论文

  • 发表日期：

    2025年3月28日

查看全文
  • Jing-yi Zhao,
  • Fan Gao,
  • Mengru Wu,
  • Yang Li,
  • Yong Chen,
  • Zijun Xiao

摘要

Enantiopure (S)-2-methylbutanoic acid [(S)-2-MBA] is a high-value chiral compound with applications in fragrances, pharmaceuticals, and agrochemicals. However, conventional chemical synthesis lacks stereoselectivity, while existing biosynthetic methods suffer from low yield and purity. Here, we report a novel microbial process using Bacillus spizizenii ATCC 6633 for efficient (S)-2-MBA production via L-isoleucine catabolism. Through targeted screening of rhizospheric soil isolates and Bacillaceae strains, ATCC 6633 demonstrated superior performance, producing 3.67 g/L (S)-2-MBA with 99.32% enantiomeric excess (ee) under optimized conditions (45 °C, 8% inoculation, 5 g/L glucose, and 8 g/L L-isoleucine). A 58.92% conversion efficiency was achieved, and a simplified purification process recovered 63.90% product with 97.32% purity. Mechanistic studies suggested glucose depletion triggered (S)-2-MBA accumulation, aligning with starvation-induced secondary metabolism. This cost-effective, eco-friendly approach eliminates racemic separation steps and harsh reagents, positioning ATCC 6633 as a promising biocatalyst for sustainable (S)-2-MBA production.