Bio-Synthesized Silver Nanoparticles from Neolamarckia Cadamba: Characterization and Analysis
摘要
Nanotechnology has brought about a notable revolution in the plant-assisted production of nanoparticles, owing to its distinctive attributes such as rapid synthesis, low toxicity, simplicity, environmentally friendly nature and cost-effectiveness. The current investigation successfully synthesized silver nanoparticles using Neolamarckia cadamba leaf extract. Characterization of bio-synthesized nanoparticles with microscopic, spectroscopic, X-ray and diffractometric techniques confirmed the crystallinity of product with irregular morphology and an average particle size ranging from 50 to 100 nm along with 97% weight percentage of silver, indicating highly efficient reduction of silver ions facilitated by the bioactive components of N. cadamba leaf extract, and functional group demonstrating a strong binding affinity of proteins to metallic silver. Alongside, the antimicrobial activity of synthesized silver nanoparticles was assessed against human pathogens such as E. coli, P. aeruginosa, K. pneumoniae, and S. aureus with variable concentrations to investigate its potential for commercial utility. The largest inhibition zones were investigated as 7.5 mm for E. coli, followed by 7.0 mm for P. aeruginosa, 6.0 mm for K. pneumoniae and 4.5 mm for S. aureus, revealing higher sensitivity of E. coli attributed to more effective interaction of Nc-AgNPs with its cell envelope, leading to enhanced membrane permeability and subsequent cellular damage.