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<p>In the high-solid anaerobic digestion (HSAD) of kitchen waste, volatile fatty acids (VFAs) tend to accumulate and further cause system acidification, of which pH is a core indicator affecting acidification degree. In this study, a one-time pH adjustment strategy was used to investigate the effects of different pH adjustments on the HSAD of kitchen waste. The group with one-time pH adjusted to 9 on the first day achieved a cumulative methane production rate of 100.3 ± 10.3 mL/g VS, which was 92.6% and 60.8% higher than that of pH7 (52.1 ± 8.5 mL/g VS) and pH8 (62.4 ± 4.3 mL/g VS), respectively. In contrast, methane production failed to recover in both the control and pH6 group. Microbial community analysis revealed that the one-time pH adjustment to 9 on day 1 effectively increased the relative abundance of <i>Syntrophomonas</i> and alleviated the accumulation of VFAs. Metagenomic analysis further demonstrated that one-time pH adjustment to 9 strengthened methane metabolic pathways. Meanwhile, the relative abundance of genes coding ATPases and ABC transport system were upregulated, which may supply sufficient energy for microbial metabolism and nutrient uptake. This study provides valuable experience for alleviating the acidification in the HSAD of kitchen waste.</p>

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Metagenomic Insights into Alkaline pH Adjustment as a Strategic Control for Enhancing Methane Production in High-Solid Anaerobic Digestion of Kitchen Waste

  • 期刊论文

  • 发表日期：

    2026年6月25日

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  • Zhen Wang,
  • Xinyi Zhang,
  • Yuming Xu,
  • Yu Xie,
  • Shihao Zhang

摘要

In the high-solid anaerobic digestion (HSAD) of kitchen waste, volatile fatty acids (VFAs) tend to accumulate and further cause system acidification, of which pH is a core indicator affecting acidification degree. In this study, a one-time pH adjustment strategy was used to investigate the effects of different pH adjustments on the HSAD of kitchen waste. The group with one-time pH adjusted to 9 on the first day achieved a cumulative methane production rate of 100.3 ± 10.3 mL/g VS, which was 92.6% and 60.8% higher than that of pH7 (52.1 ± 8.5 mL/g VS) and pH8 (62.4 ± 4.3 mL/g VS), respectively. In contrast, methane production failed to recover in both the control and pH6 group. Microbial community analysis revealed that the one-time pH adjustment to 9 on day 1 effectively increased the relative abundance of Syntrophomonas and alleviated the accumulation of VFAs. Metagenomic analysis further demonstrated that one-time pH adjustment to 9 strengthened methane metabolic pathways. Meanwhile, the relative abundance of genes coding ATPases and ABC transport system were upregulated, which may supply sufficient energy for microbial metabolism and nutrient uptake. This study provides valuable experience for alleviating the acidification in the HSAD of kitchen waste.