<p>A self-made equipment was used to study the electrokinetic remediation of copper-contaminated soil. The speciation transformation rules of copper (Cu) at different remediation times and voltages were explored. Experimental parameters such as electrolyte pH value, soil pH value, soil moisture content, and soil conductivity were measured and analyzed. The Community Bureau of Reference (BCR) sequential extraction procedure was applied to measure the speciation of Cu in soil. Results showed that after the electrokinetic remediation, the experimental parameters changed to varying degrees, which had a certain impact on the removal and transformation of Cu in the soil. The acidic region in the soil was more conducive to the speciation transformation of Cu than the alkaline region. Higher moisture content and larger electrical current accelerated the migration of ions in the soil. Results of the BCR sequential extraction method revealed that the ratio of EX-Cu content increased from 37.50% to 50.60%, the ratio of RED-Cu content decreased from 12.25% to 10.50%. The proportion of OXI-Cu content initially increased and then decreased, while the ratio of RES-Cu content decreased from the anode to the cathode. However, due to the stable properties of OXI-Cu and RES-Cu, the proportions of OXI-Cu and RES-Cu changed minimally during the process of remediation.</p>