<p>This study investigated the combined effect of biochar (derived from rice husk and horse manure) and plant-growth-promoting rhizobacteria (PGPR) on the biochemical and yield characteristics of dill (<i>Anethum graveolens</i> L.). A greenhouse experiment was conducted using six treatments: T1 (control; no biochar or PGPR), T2 (PGPR only), T3 (rice husk biochar, RHB), T4 (RHB + PGPR), T5 (horse manure biochar, HMB), and T6 (HMB + PGPR), each in triplicate. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM–EDS) was used to characterize the morphological and elemental composition of the biochar, confirming its suitability for agricultural use. The incorporation of biochar and PGPR significantly (<i>p</i> &lt; 0.05) enhanced the biochemical and yield parameters of dill plants. The T6 treatment yielded the highest values for seedling emergence (95.12%), seedling fresh biomass (3.27&#xa0;g), seedling length (8.50&#xa0;cm), root length (5.14&#xa0;cm), total chlorophyll content (0.58&#xa0;mg/g), total carotenoids (0.21&#xa0;mg/g fresh weight), total flavonoids (110&#xa0;mg/g), total phenols (128&#xa0;mg/g fresh weight), ascorbic acid (0.31&#xa0;µg/g), coumarins (0.14&#xa0;µg/g), and α-phellandrene (152.75&#xa0;µg/g). However, the highest catalase (CAT; 61.44&#xa0;µg/g) and superoxide dismutase (SOD; 97.30&#xa0;µg/g) activities were observed in the T5 treatment. Yield parameters, including seed yield per plant (26.93&#xa0;g), plant height (135.19&#xa0;cm), number of branches per plant (19.00), number of umbels per plant (44.00), and number of umbellets per umbel (19.00), were highest in the T6 treatment. Analysis of variance (ANOVA) followed by Tukey’s post hoc test confirmed the statistically significant (<i>p</i> &lt; 0.05) improvement in biochemical and yield responses due to biochar and PGPR application. These results highlight the potential of integrating agricultural and livestock waste-derived biochar with PGPR for sustainable crop production.</p>

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Synergistic Effects of Waste Derived Biochar and PGPR on the Biochemical and Yield Attributes of Dill (Anethum graveolens L.)

  • Mohssen Elbagory,
  • Nagwa EL-Khateeb,
  • Sahar El-Nahrawy,
  • Alaa El-Dein Omara,
  • Ibrahim Mohamed,
  • Marwa Yasien Helmy Elbyaly,
  • Mahmoud El-Sharkawy,
  • Amal Zayed,
  • Ivan Širić,
  • Pankaj Kumar

摘要

This study investigated the combined effect of biochar (derived from rice husk and horse manure) and plant-growth-promoting rhizobacteria (PGPR) on the biochemical and yield characteristics of dill (Anethum graveolens L.). A greenhouse experiment was conducted using six treatments: T1 (control; no biochar or PGPR), T2 (PGPR only), T3 (rice husk biochar, RHB), T4 (RHB + PGPR), T5 (horse manure biochar, HMB), and T6 (HMB + PGPR), each in triplicate. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM–EDS) was used to characterize the morphological and elemental composition of the biochar, confirming its suitability for agricultural use. The incorporation of biochar and PGPR significantly (p < 0.05) enhanced the biochemical and yield parameters of dill plants. The T6 treatment yielded the highest values for seedling emergence (95.12%), seedling fresh biomass (3.27 g), seedling length (8.50 cm), root length (5.14 cm), total chlorophyll content (0.58 mg/g), total carotenoids (0.21 mg/g fresh weight), total flavonoids (110 mg/g), total phenols (128 mg/g fresh weight), ascorbic acid (0.31 µg/g), coumarins (0.14 µg/g), and α-phellandrene (152.75 µg/g). However, the highest catalase (CAT; 61.44 µg/g) and superoxide dismutase (SOD; 97.30 µg/g) activities were observed in the T5 treatment. Yield parameters, including seed yield per plant (26.93 g), plant height (135.19 cm), number of branches per plant (19.00), number of umbels per plant (44.00), and number of umbellets per umbel (19.00), were highest in the T6 treatment. Analysis of variance (ANOVA) followed by Tukey’s post hoc test confirmed the statistically significant (p < 0.05) improvement in biochemical and yield responses due to biochar and PGPR application. These results highlight the potential of integrating agricultural and livestock waste-derived biochar with PGPR for sustainable crop production.