Scholarlink

<p>Petroleum Hydrocarbons are hazardous pollutants to the environment and have toxic effects on plants and animals including humans. It alters the biological properties of soil by affecting plant growth, microbial diversity, and their enzymatic activities. It has a complex structure that persists very long time in the environment and is difficult to degrade. Various chemical and physical techniques have been operated for the remediation of petroleum hydrocarbon (PHC) contaminated soil but they are very expensive and also produce secondary pollutants during the treatment. To overcome this problem, researchers develop environmentally friendly and low-cost techniques i.e. bioremediation for the degradation of petroleum hydrocarbons. This research focused on isolating hydrocarbon-degrading bacteria and evaluating their effectiveness in petroleum hydrocarbon degradation. Thirty nine bacteria have been isolated from PHC soil samples and were screened at 1% to 5% crude oil concentration (v/v) with DCPIP redox indicator in Bushnell Hass medium. Among the positive screening isolates, nine of the best bacteria were selected to study their degradation efficacy. The degradation rates of nine different bacterial isolates ranged from 41.06 ± 4.6% to 83.14 ± 3.18% over 14&#xa0;days at 1% v/v crude oil concentration. The bacterial strain MG4A-1 (<i>Micrococcus sp</i>.) shows the least degradation capacity, while strain MG1B-13 (<i>Azotobacter sp</i>.) exhibits the highest degradation capacity. This study has identified effective bacterial strains capable of degrading petroleum hydrocarbons, providing a foundation for developing successful bioremediation strategies in petroleum contaminated environments.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Isolation, Screening, and Biodegradation of Crude Oil by Indigenous Bacteria Isolated from Petroleum Hydrocarbon-Contaminated Soil

  • 期刊论文

  • 发表日期：

    2025年8月7日

查看全文
  • Salim Ahmed,
  • Durgeshwer Singh

摘要

Petroleum Hydrocarbons are hazardous pollutants to the environment and have toxic effects on plants and animals including humans. It alters the biological properties of soil by affecting plant growth, microbial diversity, and their enzymatic activities. It has a complex structure that persists very long time in the environment and is difficult to degrade. Various chemical and physical techniques have been operated for the remediation of petroleum hydrocarbon (PHC) contaminated soil but they are very expensive and also produce secondary pollutants during the treatment. To overcome this problem, researchers develop environmentally friendly and low-cost techniques i.e. bioremediation for the degradation of petroleum hydrocarbons. This research focused on isolating hydrocarbon-degrading bacteria and evaluating their effectiveness in petroleum hydrocarbon degradation. Thirty nine bacteria have been isolated from PHC soil samples and were screened at 1% to 5% crude oil concentration (v/v) with DCPIP redox indicator in Bushnell Hass medium. Among the positive screening isolates, nine of the best bacteria were selected to study their degradation efficacy. The degradation rates of nine different bacterial isolates ranged from 41.06 ± 4.6% to 83.14 ± 3.18% over 14 days at 1% v/v crude oil concentration. The bacterial strain MG4A-1 (Micrococcus sp.) shows the least degradation capacity, while strain MG1B-13 (Azotobacter sp.) exhibits the highest degradation capacity. This study has identified effective bacterial strains capable of degrading petroleum hydrocarbons, providing a foundation for developing successful bioremediation strategies in petroleum contaminated environments.