Scholarlink

Background <p>Biogas slurry (BGS), a byproduct of anaerobic digestion of animal manure and agricultural residues, is recognized as a high-quality bio-organic fertilizer. Plant growth-promoting rhizobacteria (PGPR) are well known for enhancing metal stress tolerance in plants. The combined application of PGPR and BGS may have the potential to modulate nickel (Ni) toxicity, bioaccumulation, and plant physiology.</p> Purpose and Methods <p>The purpose of this study was to reduce physiological stress in radish (<i>Raphanus sativus</i> L.) by regulating Ni uptake and antioxidant defense systems through inoculation of <i>Bacillus</i> sp. CIK-512 along with BGS. <i>Bacillus</i> sp. CIK-512 was applied alone and in combination with BGS to radish plants subjected to 40&#xa0;mg&#xa0;kg<sup>−1</sup> of Ni content in a pot experiment.</p> Results <p>Integrated use of <i>Bacillus</i> sp. CIK-512 and BGS significantly increased biomass by 15% and 80%, relative water contents by 19% and 18%, photosynthetic rate by 20% and 32%, and stomatal conductance by 12% and 34% of radish plants under normal and Ni stress conditions, respectively. Root accumulated a comparatively higher amount of Ni than shoot. Lipid peroxidation was repaired by the radish plants through enhanced activities of ascorbate peroxidase and catalase antioxidants, however, <i>Bacillus</i> sp. CIK-512 and BGS modulated these parameters to mitigate Ni stress.</p> Conclusion <p>This study concluded that Ni stress could be mitigated through the application of <i>Bacillus</i> sp. CIK-512 and BGS, which lowers Ni accumulation in radish roots and shoots, modulates the oxidative damage and antioxidant defense system in radish plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Bacillus Inoculation Along with Biogas Slurry Mitigates Nickel Stress in Raphanus Sativus by Regulating Ni Accumulation and the Antioxidant Defense System

  • 期刊论文

  • 发表日期：

    2025年7月21日

查看全文
  • Iftikhar Ahmad,
  • Shehzad Mehmood,
  • Muhammad Atif Muneer,
  • Aftab Jamal,
  • Amjad Farooq,
  • Muhammad Farhan Saeed,
  • Qasim Ali,
  • Umar Daraz,
  • Nabil Touzout,
  • Adil Mihoub,
  • Jun Zhou

摘要

Background

Biogas slurry (BGS), a byproduct of anaerobic digestion of animal manure and agricultural residues, is recognized as a high-quality bio-organic fertilizer. Plant growth-promoting rhizobacteria (PGPR) are well known for enhancing metal stress tolerance in plants. The combined application of PGPR and BGS may have the potential to modulate nickel (Ni) toxicity, bioaccumulation, and plant physiology.

Purpose and Methods

The purpose of this study was to reduce physiological stress in radish (Raphanus sativus L.) by regulating Ni uptake and antioxidant defense systems through inoculation of Bacillus sp. CIK-512 along with BGS. Bacillus sp. CIK-512 was applied alone and in combination with BGS to radish plants subjected to 40 mg kg−1 of Ni content in a pot experiment.

Results

Integrated use of Bacillus sp. CIK-512 and BGS significantly increased biomass by 15% and 80%, relative water contents by 19% and 18%, photosynthetic rate by 20% and 32%, and stomatal conductance by 12% and 34% of radish plants under normal and Ni stress conditions, respectively. Root accumulated a comparatively higher amount of Ni than shoot. Lipid peroxidation was repaired by the radish plants through enhanced activities of ascorbate peroxidase and catalase antioxidants, however, Bacillus sp. CIK-512 and BGS modulated these parameters to mitigate Ni stress.

Conclusion

This study concluded that Ni stress could be mitigated through the application of Bacillus sp. CIK-512 and BGS, which lowers Ni accumulation in radish roots and shoots, modulates the oxidative damage and antioxidant defense system in radish plants.