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<p>A novel lytic phage with a broad host range was isolated from pig faeces and the complete genome was subsequently sequenced. The phage was found to lyse <i>Staphylococcus hyicus</i>, <i>S. pseudintermedius</i>, <i>S. schleiferi</i> and <i>S. warneri</i>, generating approximately 27 PFU per infected <i>S. hyicus</i> cell. The phage has an isometric head of 42 ± 2 nm in diameter and a noncontractile tail of 114 ± 9 nm long. The genome is 53,660 bp in size and consists of 79 predicted ORFs and one tRNA<sup>Arg</sup> gene. The phage has been classified within the Caudoviricetes, specifically the Chaseviridae family. Its broad host range and absence of harmful genes make it suitable for use in phage therapy. In addition, a novel temperate phage was discovered that was spontaneously released from a <i>S. hyicus</i> isolate Pel11 from a pig with exudative epidermitis. This novel temperate phage differs from the known temperate phages in <i>S. hyicus</i> strains NCTC10350, MM2101 or 83/7-1B, representing a novel pathogenicity element in the <i>S. hyicus</i> genome.</p>

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New lytic and new temperate Staphylococcus hyicus phages

  • 期刊论文

  • 发表日期：

    2025年3月14日

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  • Karel Petrzik,
  • Lucie Sovová

摘要

A novel lytic phage with a broad host range was isolated from pig faeces and the complete genome was subsequently sequenced. The phage was found to lyse Staphylococcus hyicus, S. pseudintermedius, S. schleiferi and S. warneri, generating approximately 27 PFU per infected S. hyicus cell. The phage has an isometric head of 42 ± 2 nm in diameter and a noncontractile tail of 114 ± 9 nm long. The genome is 53,660 bp in size and consists of 79 predicted ORFs and one tRNAArg gene. The phage has been classified within the Caudoviricetes, specifically the Chaseviridae family. Its broad host range and absence of harmful genes make it suitable for use in phage therapy. In addition, a novel temperate phage was discovered that was spontaneously released from a S. hyicus isolate Pel11 from a pig with exudative epidermitis. This novel temperate phage differs from the known temperate phages in S. hyicus strains NCTC10350, MM2101 or 83/7-1B, representing a novel pathogenicity element in the S. hyicus genome.