Scholarlink

<p>Wild animals often play a role in the transmission of zoonotic pathogens. Among the wild mammals of South America, ring-tailed coatis (<i>Nasua nasua</i>) can present a high frequency of anti-<i>Leptospira</i> antibodies and carry <i>Leptospira</i> spp. This study aimed to evaluate the frequency of serum anti-<i>Leptospira</i> antibodies and perform the molecular identification of <i>Leptospira</i> spp. in ring-tailed coatis living in the Tietê River Ecological Park, in southeastern Brazil. Blood and urine samples were obtained from ring-tailed coatis. The serum was tested using the Microscopic Agglutination Test (MAT). Urine was cultured in semi-solid Ellinghausen–McCullough–Johnson–Harris medium. The inoculum was cultivated at 28&#xa0;°C and monitored over six weeks. Total DNA from the blood and urine samples was extracted, and the 16&#xa0;S rRNA gene and <i>lipL32</i> were searched by PCR. Of 192 animals, 36 (18.75%) had positive serum samples in the MAT. The most frequent serogroups in MAT were Grippotyphosa (11.46%; 22/192), Autumnalis (7.81%; 15/192) and Cynopteri (2.60%; 5/192). A total of 116 urine samples were obtained. None of the <i>Leptospira</i> spp. were isolated, twelve samples were positive for the 16&#xa0;S rRNA gene (10.34%), and four were positive for the <i>lipL32</i> gene (3.4%). These results indicate a high frequency of serum anti-<i>Leptospira</i> spp. antibodies in coatis from the Tietê River Ecological Park and highlight the possibility of their potential role as carriers of pathogenic <i>Leptospira</i> spp. in urine.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Serological and molecular detection of Leptospira spp. in ring-tailed coatis (Nasua nasua) from an Ecological Park

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月1日

查看全文
  • Gabriel Siqueira dos Santos,
  • Denise Batista Nogueira Silva,
  • Israel Barbosa Guedes,
  • Rafael Rodrigues Soares,
  • Nathália da Silveira Guimarães,
  • Gisele Oliveira de Souza,
  • Bruno Simões Sergio Petri,
  • Haroldo Furuya,
  • Ricardo Augusto Dias,
  • José Soares Ferreira Neto,
  • Marcos Bryan Heinemann

摘要

Wild animals often play a role in the transmission of zoonotic pathogens. Among the wild mammals of South America, ring-tailed coatis (Nasua nasua) can present a high frequency of anti-Leptospira antibodies and carry Leptospira spp. This study aimed to evaluate the frequency of serum anti-Leptospira antibodies and perform the molecular identification of Leptospira spp. in ring-tailed coatis living in the Tietê River Ecological Park, in southeastern Brazil. Blood and urine samples were obtained from ring-tailed coatis. The serum was tested using the Microscopic Agglutination Test (MAT). Urine was cultured in semi-solid Ellinghausen–McCullough–Johnson–Harris medium. The inoculum was cultivated at 28 °C and monitored over six weeks. Total DNA from the blood and urine samples was extracted, and the 16 S rRNA gene and lipL32 were searched by PCR. Of 192 animals, 36 (18.75%) had positive serum samples in the MAT. The most frequent serogroups in MAT were Grippotyphosa (11.46%; 22/192), Autumnalis (7.81%; 15/192) and Cynopteri (2.60%; 5/192). A total of 116 urine samples were obtained. None of the Leptospira spp. were isolated, twelve samples were positive for the 16 S rRNA gene (10.34%), and four were positive for the lipL32 gene (3.4%). These results indicate a high frequency of serum anti-Leptospira spp. antibodies in coatis from the Tietê River Ecological Park and highlight the possibility of their potential role as carriers of pathogenic Leptospira spp. in urine.