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<p>Oral daily probiotic and postbiotic supplementation (ODPPS) is a promising strategy for canine skin and gut health. Despite growing interest, the effects of ODPPS on the composition of the canine gut and skin microbiota remain largely unexplored. The advent of full-length 16S rRNA gene sequencing has opened new avenues in microbiome research, significantly enhancing the accuracy and completeness of microbial community profiling. This study employed PacBio long-read sequencing to profile longitudinal changes in canine fecal microbiota composition during ODPPS. Then, we evaluated the parallel dynamics between fecal and skin microbiota responses to ODPPS, addressing a critical gap in understanding systemic microbiome interactions in dogs. By Day 90, fecal microbiota composition shifted significantly (PERMANOVA <i>p</i> = 0.05), with increased beneficial bacteria by Day 30 and further by Day 90. In both axillae and fecal microbiota, ODPPS supplementation increased the relative abundance of beneficial species including <i>Lactobacillus acidophilus</i>, <i>Lactobacillus johnsonii</i>, and <i>Limosilactobacillus reuteri</i>. A significant positive Pearson correlation was found between the relative abundances of these bacteria in the skin and fecal microbiota (<i>p</i> = 0.05), suggesting a coordinated microbial response across body sites. The results highlight the significance of <i>Lactobacillus acidophilus</i> as a core probiotic strain, demonstrating exceptional capacity for colonisation and establishment in both the gastrointestinal and skin niches of dogs. These findings show the systemic influence of ODPPS in shaping microbial communities across both sites and support its use to promote overall canine health. Collectively, our results offer robust evidence for the dual benefits of daily oral ODPPS on both skin and gut microbiota.</p>

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Oral probiotic and postbiotic supplementation enhances the abundance of Lactobacillus acidophilus, Lactobacillus johnsonii, and Limosilactobacillus reuteri in both canine skin and gastrointestinal microbiota: insights from long-read 16S rRNA gene sequencing

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Manijeh Mohammadi Dehcheshmeh,
  • Letitia Grant,
  • Esmaeil Ebrahimie,
  • Aliakbar Khabiri,
  • Farhid Hemmatzadeh,
  • Michael Shipstone,
  • Darren J. Trott

摘要

Oral daily probiotic and postbiotic supplementation (ODPPS) is a promising strategy for canine skin and gut health. Despite growing interest, the effects of ODPPS on the composition of the canine gut and skin microbiota remain largely unexplored. The advent of full-length 16S rRNA gene sequencing has opened new avenues in microbiome research, significantly enhancing the accuracy and completeness of microbial community profiling. This study employed PacBio long-read sequencing to profile longitudinal changes in canine fecal microbiota composition during ODPPS. Then, we evaluated the parallel dynamics between fecal and skin microbiota responses to ODPPS, addressing a critical gap in understanding systemic microbiome interactions in dogs. By Day 90, fecal microbiota composition shifted significantly (PERMANOVA p = 0.05), with increased beneficial bacteria by Day 30 and further by Day 90. In both axillae and fecal microbiota, ODPPS supplementation increased the relative abundance of beneficial species including Lactobacillus acidophilus, Lactobacillus johnsonii, and Limosilactobacillus reuteri. A significant positive Pearson correlation was found between the relative abundances of these bacteria in the skin and fecal microbiota (p = 0.05), suggesting a coordinated microbial response across body sites. The results highlight the significance of Lactobacillus acidophilus as a core probiotic strain, demonstrating exceptional capacity for colonisation and establishment in both the gastrointestinal and skin niches of dogs. These findings show the systemic influence of ODPPS in shaping microbial communities across both sites and support its use to promote overall canine health. Collectively, our results offer robust evidence for the dual benefits of daily oral ODPPS on both skin and gut microbiota.