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<p>The aim of this study is to explore the protective effect of selenomethionine (SeMet) against the immunotoxicity caused by aflatoxin B1 (AFB1) in male rabbits. Fifty healthy male rabbits aged 90 days were randomly divided into 5 groups: control group, model group, low dose selenomethionine group (Se-1), middle dose selenomethionine group (Se-2) and high dose selenomethionine group (Se-3). The control group and model group were fed basal diet, and the other three groups were fed SeMet 0.2&#xa0;mg/kg, 0.4&#xa0;mg/kg and 0.6&#xa0;mg/kg respectively. On the 31st day of the experiment, All rabbits in the model group and three treatment groups were fed 0.5&#xa0;mg/kg AFB1 for 21 days. Detect the levels of immune-related indicators in rabbit plasma. Collect samples, and observe the pathological changes in rabbit spleen and sacculus rotundus using hematoxylin-eosin (HE) staining. Analyze the expression of related proteins in spleen and sacculus rotundus tissues using immunofluorescence and Western blot (WB) analysis. Measure the levels of relevant inflammatory factor mRNA in spleen and sacculus rotundus tissues using qRT-PCR. Use enzyme-linked immunosorbent assay (ELISA) to detect the levels of oxidative stress-related indicators and inflammatory factors in spleen and sacculus rotundus tissues. The results indicate that AFB1 can induce oxidative stress and inflammation by activating the TLR-2/NF-κB signaling pathway, inhibiting the proliferation and differentiation of spleen and bursa cells, disrupting the normal structure of the spleen and sacculus rotundus tissues, and reducing the levels of immune-related indicators in plasma. SeMet pretreatment significantly inhibited the TLR-2/NF-κB signaling pathway, alleviating oxidative stress damage in spleen and sacculus rotundus tissues and reducing the inflammatory response in rabbits.Thus, we demonstrate that SeMet mitigates AFB1-induced immunotoxicity in reserve male rabbits by inhibiting the TLR-2/NF-κB signaling pathway. In addition, in this study, the effect of 0.4&#xa0;mg/kg SeMet was the most effective. SeMet is a natural and safe drug that can prevent AFB1 poisoning in rabbit feed.</p>

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Protective effect of selenomethionine on spleen and sacculus rotundus injury induced by aflatoxin B1 in reserve male rabbits

  • 期刊论文

  • 发表日期：

    2025年10月28日

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  • Zhe Zhang,
  • Shiyang Liu,
  • Yumei Liu,
  • Chaoying Zhang,
  • Zihao Meng,
  • Qiongxia Lv,
  • Dongliu Luo,
  • Xiaoguang Chen,
  • Xuemin Zhu,
  • Ziqiang Zhang

摘要

The aim of this study is to explore the protective effect of selenomethionine (SeMet) against the immunotoxicity caused by aflatoxin B1 (AFB1) in male rabbits. Fifty healthy male rabbits aged 90 days were randomly divided into 5 groups: control group, model group, low dose selenomethionine group (Se-1), middle dose selenomethionine group (Se-2) and high dose selenomethionine group (Se-3). The control group and model group were fed basal diet, and the other three groups were fed SeMet 0.2 mg/kg, 0.4 mg/kg and 0.6 mg/kg respectively. On the 31st day of the experiment, All rabbits in the model group and three treatment groups were fed 0.5 mg/kg AFB1 for 21 days. Detect the levels of immune-related indicators in rabbit plasma. Collect samples, and observe the pathological changes in rabbit spleen and sacculus rotundus using hematoxylin-eosin (HE) staining. Analyze the expression of related proteins in spleen and sacculus rotundus tissues using immunofluorescence and Western blot (WB) analysis. Measure the levels of relevant inflammatory factor mRNA in spleen and sacculus rotundus tissues using qRT-PCR. Use enzyme-linked immunosorbent assay (ELISA) to detect the levels of oxidative stress-related indicators and inflammatory factors in spleen and sacculus rotundus tissues. The results indicate that AFB1 can induce oxidative stress and inflammation by activating the TLR-2/NF-κB signaling pathway, inhibiting the proliferation and differentiation of spleen and bursa cells, disrupting the normal structure of the spleen and sacculus rotundus tissues, and reducing the levels of immune-related indicators in plasma. SeMet pretreatment significantly inhibited the TLR-2/NF-κB signaling pathway, alleviating oxidative stress damage in spleen and sacculus rotundus tissues and reducing the inflammatory response in rabbits.Thus, we demonstrate that SeMet mitigates AFB1-induced immunotoxicity in reserve male rabbits by inhibiting the TLR-2/NF-κB signaling pathway. In addition, in this study, the effect of 0.4 mg/kg SeMet was the most effective. SeMet is a natural and safe drug that can prevent AFB1 poisoning in rabbit feed.