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<p><i>Clostridium perfringens</i> (<i>C. perfringens</i>) is one of the major pathogens responsible for clostridial diarrhea in animals, especially young animals under stress, leading to increased mortality and economic losses. With increasing resistance to antibiotics, phages and phage-derived endolysins have been considered promising alternatives for combating antibiotic-resistant bacteria. In this study, we expressed and purified the endolysin (LysDCp1, NCBI accession no. UXL90914.1) encoded by phage DCp1, and evaluated its lytic activity against <i>C. perfringens</i> and in vivo bactericidal efficacy using <i>Galleria mellonella</i> larvae. The results showed that LysDCp1 exhibited a higher lytic activity against <i>C. perfringens</i> and a wider lytic spectrum than phage DCp1. LysDCp1 dramatically reduced the concentration of <i>C. perfringens</i> D14 in 8&#xa0;h, with a minimum Inhibitory Concentration (MIC) of 12.5&#xa0;µg/mL. LysDCp1 displayed the optimal bactericidal activity at pH 7 and moderate temperatures (20–40℃).LysDCp1 exhibited high resistance to high temperatures and alkaline conditions, but it readily lost activity in acidic environments. LysDCp1 remarkably eliminated <i>C. perfringens</i> D14 biofilms in 3&#xa0;h. Moreover, LysDCp1 significantly reduced the number of <i>C. perfringens</i> in the hemolymph of <i>C. perfringens</i>-challenged <i>G. mellonella</i> larvae and improved their survival. The findings highlight the great potential of LysDCp1 as an effective therapeutic agent in treating clostridial diarrhea caused by <i>C. perfringens</i> in animals.</p>

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High lytic activity of endolysin LysDCp1 from phage DCp1 against Clostridium perfringens and biofilms

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Ruixue Ma,
  • Zhaohui Tang,
  • Jianchao Zhao,
  • Ziyang Li,
  • Yanshun Cui,
  • Can Zhang,
  • Huiying Ren,
  • Shouzhen Xu,
  • Ling Zou,
  • Jing Ma,
  • Jianbao Dong,
  • Xiaojing Hao,
  • Wenhua Liu

摘要

Clostridium perfringens (C. perfringens) is one of the major pathogens responsible for clostridial diarrhea in animals, especially young animals under stress, leading to increased mortality and economic losses. With increasing resistance to antibiotics, phages and phage-derived endolysins have been considered promising alternatives for combating antibiotic-resistant bacteria. In this study, we expressed and purified the endolysin (LysDCp1, NCBI accession no. UXL90914.1) encoded by phage DCp1, and evaluated its lytic activity against C. perfringens and in vivo bactericidal efficacy using Galleria mellonella larvae. The results showed that LysDCp1 exhibited a higher lytic activity against C. perfringens and a wider lytic spectrum than phage DCp1. LysDCp1 dramatically reduced the concentration of C. perfringens D14 in 8 h, with a minimum Inhibitory Concentration (MIC) of 12.5 µg/mL. LysDCp1 displayed the optimal bactericidal activity at pH 7 and moderate temperatures (20–40℃).LysDCp1 exhibited high resistance to high temperatures and alkaline conditions, but it readily lost activity in acidic environments. LysDCp1 remarkably eliminated C. perfringens D14 biofilms in 3 h. Moreover, LysDCp1 significantly reduced the number of C. perfringens in the hemolymph of C. perfringens-challenged G. mellonella larvae and improved their survival. The findings highlight the great potential of LysDCp1 as an effective therapeutic agent in treating clostridial diarrhea caused by C. perfringens in animals.