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<p>The gastrointestinal health of livestock is essential for the agricultural sector. Rutin, a natural flavonoid extract from plants, exhibits antioxidant and anti-inflammatory properties, alongside a protective impact on the intestinal tract of pigs. However its influence on the proliferation, migration, and cellular junctions of IPEC-J2 cells remains unexplored. This study involved treating IPEC-J2 cells with varying concentrations of rutin. It was observed that after 24&#xa0;h of treatment with 200 µM rutin, cell viability was significantly increased, along with significant upregulation in the mRNA expression levels of proliferation-related genes (<i>CCNE2</i>, <i>CDK4</i>,<i> CDK2</i>,<i> CCNDB</i>,<i> and PCNA</i>) and tight junction genes (<i>ZO-1</i>, <i>Occludin</i>, and <i>Claudin-1</i>). Additionally, the expression of proliferation-related PCNA protein was significantly increased. Transcriptome sequencing revealed that rutin treatment impacted the overall transcription profile of IPEC-J2 cells, altering the expression of genes involved in critical physiological processes such as redox balance, cell proliferation, and tight junctions maintenance. Consequently, rutin demonstrated a significant enhancement in the proliferation of IPEC-J2 cells and improved the intestinal barrier function in pigs, offering valuable insights for sustaining pig intestinal health in swine husbandry.</p>

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Transcriptomic analysis reveals the impact of Rutin on the proliferation, barrier function, and transcription of Porcine IPEC-J2

  • 期刊论文

  • 发表日期：

    2025年8月15日

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  • Binbin Wang,
  • Lele Huo,
  • Wanru Tian,
  • Wenwen Xing,
  • Gang Luo,
  • Mengxuan Li,
  • Liumei Sun,
  • Jiying Liu

摘要

The gastrointestinal health of livestock is essential for the agricultural sector. Rutin, a natural flavonoid extract from plants, exhibits antioxidant and anti-inflammatory properties, alongside a protective impact on the intestinal tract of pigs. However its influence on the proliferation, migration, and cellular junctions of IPEC-J2 cells remains unexplored. This study involved treating IPEC-J2 cells with varying concentrations of rutin. It was observed that after 24 h of treatment with 200 µM rutin, cell viability was significantly increased, along with significant upregulation in the mRNA expression levels of proliferation-related genes (CCNE2, CDK4, CDK2, CCNDB, and PCNA) and tight junction genes (ZO-1, Occludin, and Claudin-1). Additionally, the expression of proliferation-related PCNA protein was significantly increased. Transcriptome sequencing revealed that rutin treatment impacted the overall transcription profile of IPEC-J2 cells, altering the expression of genes involved in critical physiological processes such as redox balance, cell proliferation, and tight junctions maintenance. Consequently, rutin demonstrated a significant enhancement in the proliferation of IPEC-J2 cells and improved the intestinal barrier function in pigs, offering valuable insights for sustaining pig intestinal health in swine husbandry.