Scholarlink

<p>Antimicrobial resistance in natural environments poses an increasing threat to public health and biodiversity conservation. This study investigated antimicrobial resistance and biofilm formation in bacteria isolated from <i>Inia araguaiaensis</i>, an Amazonian endemic dolphin species. Skin lesion samples were collected from eight individuals in the Mocajuba region, Pará, Brazil. Bacterial isolation was performed on Brain Heart Infusion agar and Mannitol Salt agar. Species identification was carried out using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and antimicrobial susceptibility testing was conducted by disk diffusion following. Resistance genes were detected by polymerase chain reaction, and biofilm formation was assessed using the crystal violet staining method. A total of 19 bacterial isolates were recovered, including <i>Staphylococcus</i> spp. (<i>n</i> = 14) and <i>Mammaliicoccus sciuri</i> (<i>n</i> = 5). The most frequently identified species were <i>Staphylococcus aureus</i> (4/19; 21.1%), <i>S. warneri</i> (4/19; 21.1%), and <i>S. epidermidis</i> (4/19; 21.1%). A high prevalence of penicillin resistance was observed (18/19; 94.7%), followed by resistance to erythromycin, tetracycline, and sulfamethoxazole-trimethoprim (each 4/19; 21.1%), and gentamicin (3/19; 15.8%). Four isolates (4/19; 21.1%) exhibited a multidrug-resistant (MDR) profile. Genotypic screening revealed the presence of the <i>blaZ</i> gene in 8/19 isolates (44.4%), followed by <i>mecA</i>, <i>msrA</i>, and <i>norC</i> (each 2/19; 10.5%). The tetracycline resistance genes <i>tetL</i> and <i>tetM</i> were detected in 3/19 (15.8%) and 1/19 (5.3%) isolates, respectively. Biofilm formation was detected on 15/19 (78.9%) of the isolates, of which 4/15 (26.7%) were classified as strong producers, 1/15 (6.7%) as moderate, and 10/15 (66.7%) as weak producers. These findings support the recognition of river dolphins as potential sentinels for antimicrobial resistance in aquatic ecosystems and reinforce the importance of integrating microbiological monitoring into broader conservation efforts under the One Health perspective.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Antimicrobial resistance and biofilm production by Staphylococcus spp. and Mammaliicoccus sciuri in Inia araguaiaensis: surveillance under a one health perspective in the Amazon

  • 期刊论文

  • 发表日期：

    2025年8月5日

查看全文
  • Denny Parente de Sá Barreto Maia Leite,
  • Pollyanne Raysa Fernandes de Oliveira,
  • Flavia Cristina Matos Oliveira,
  • Gabriel Melo-Santos,
  • Guilherme Maricato,
  • Karolina Rosa Fernandes Beraldo,
  • Angélica Lúcia Figueiredo Rodrigues,
  • Alexsandro Antonio Portilho Damasceno,
  • Rodrigo Hipólito Tardin Oliveira,
  • Thais Fernanda Campos Fraga-Silva,
  • Maria Aparecida Juliano,
  • Frederico Ozanan Barros Monteiro,
  • Abelardo Silva-Júnior,
  • Rinaldo Aparecido Mota

摘要

Antimicrobial resistance in natural environments poses an increasing threat to public health and biodiversity conservation. This study investigated antimicrobial resistance and biofilm formation in bacteria isolated from Inia araguaiaensis, an Amazonian endemic dolphin species. Skin lesion samples were collected from eight individuals in the Mocajuba region, Pará, Brazil. Bacterial isolation was performed on Brain Heart Infusion agar and Mannitol Salt agar. Species identification was carried out using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and antimicrobial susceptibility testing was conducted by disk diffusion following. Resistance genes were detected by polymerase chain reaction, and biofilm formation was assessed using the crystal violet staining method. A total of 19 bacterial isolates were recovered, including Staphylococcus spp. (n = 14) and Mammaliicoccus sciuri (n = 5). The most frequently identified species were Staphylococcus aureus (4/19; 21.1%), S. warneri (4/19; 21.1%), and S. epidermidis (4/19; 21.1%). A high prevalence of penicillin resistance was observed (18/19; 94.7%), followed by resistance to erythromycin, tetracycline, and sulfamethoxazole-trimethoprim (each 4/19; 21.1%), and gentamicin (3/19; 15.8%). Four isolates (4/19; 21.1%) exhibited a multidrug-resistant (MDR) profile. Genotypic screening revealed the presence of the blaZ gene in 8/19 isolates (44.4%), followed by mecA, msrA, and norC (each 2/19; 10.5%). The tetracycline resistance genes tetL and tetM were detected in 3/19 (15.8%) and 1/19 (5.3%) isolates, respectively. Biofilm formation was detected on 15/19 (78.9%) of the isolates, of which 4/15 (26.7%) were classified as strong producers, 1/15 (6.7%) as moderate, and 10/15 (66.7%) as weak producers. These findings support the recognition of river dolphins as potential sentinels for antimicrobial resistance in aquatic ecosystems and reinforce the importance of integrating microbiological monitoring into broader conservation efforts under the One Health perspective.