<p>Urbanization alters habitat structure and resource availability, reshaping the diversity and functioning of biological communities. We evaluated how urbanization influences dung beetle (Scarabaeinae) assemblages in a Neotropical Andean city (Armenia, Colombia) across three urban contexts: ex-urban, peri-urban, and intra-urban zones. Community responses were assessed using complementary measures of abundance, expressed as the number of individuals and biomass, to examine how different facets of diversity respond to urban pressure. Dung beetle assemblages exhibited contrasting responses depending on the abundance measure considered. The number of individuals differed among urbanization zones, with peri-urban areas showing higher values than exurban sites, while biomass did not vary significantly across zones, suggesting a decoupling between numerical dominance and biomass patterns. Species-level patterns revealed marked functional contrasts, with a few large-bodied species contributing disproportionately to total biomass despite low number of individuals. Diversity analyses showed higher individual-based diversity in exurban zones, whereas biomass-based diversity was higher in exurban and peri-urban areas than in intra-urban zones. Compositional dissimilarity among zones was driven primarily by species turnover, indicating that changes in community composition are mainly associated with species replacement rather than differences in richness. Our findings highlight the importance of integrating biomass with traditional abundance measures to more accurately characterize community responses to urbanization. They also emphasize the relevance of urban and periurban green spaces for sustaining functionally diverse dung beetle assemblages in Andean urban landscapes.</p>