<p>Biodiversity conservation in cities is complicated by the many anthropogenic stressors and by climate change amplification in urban environments. Low-mobility taxa sensitive to soil sealing, pollution and drought, such as soil microarthropods, are especially vulnerable to the impacts of urbanization, and urban green areas are pivotal for their preservation. When planning management actions for urban soil fauna, however, it should be considered that local, landscape and climate variables may interact in complex ways. In this study, we sampled soil microarthropods in 15 urban green areas of Bologna, Northern Italy, testing how their communities were affected by local habitat (urban parks v. urban farms), soil clay content, percentage of green areas in the landscape and seasonality (dry v. wet season). In terms of abundance and diversity, soil fauna was more sensitive to the dry season in farms than in parks. Both the percentage of green areas and soil clay content, however, were able to partially buffer the impact of the dry season, likely because of temperature mitigation and improved soil moisture, respectively. Additionally, clay reduced soil biological quality in highly urbanized landscapes, possibly due to a higher level of contaminants, whose toxicity is amplified by clay. Finally, the morphological and molecular approaches used to characterize microarthropod taxonomic richness gave markedly different results, suggesting that these methods are complementary. Overall, our results underline the importance of a widespread network of green areas for the conservation of urban soil microarthropods in a climate change context, especially when considering urban farms and clay-rich soils.</p>