Scholarlink

<p>Infectious Bovine Rhinotracheitis (IBR) is a highly contagious respiratory disease of cattle caused by <i>Bovine alphaherpesvirus</i>-1 (BoAHV-1), a member of the genus <i>Varicellovirus</i>. Similar to other alphaherpesviruses, BoAHV-1 establishes lifelong latency with the potential for reactivation under immunosuppressive or stressful conditions. The viral envelope contains several glycoproteins, among which gB, gC, and gD are the major immunogens recognized by sera of infected cattle. Glycoprotein B (gB) is highly conserved and strongly immunogenic, making it a suitable target for diagnostic assay development. In this study, a truncated <i>gB</i> fragment was cloned and expressed in <i>Escherichia coli</i>. The recombinant gB (rgB) (~ 47&#xa0;kDa) was purified under denaturing conditions using Ni-NTA affinity chromatography and confirmed by SDS-PAGE and western blotting. The protein showed strong reactivity with anti-His antibodies and IBR-positive bovine sera, indicating preserved immunoreactive epitopes. The purified protein was standardized as a coating antigen in an indirect ELISA (iELISA). Diagnostic evaluation using 162 sera, compared with the ID Screen<sup>®</sup> IBR gB Competition ELISA, yielded a ROC-determined cut-off OD of 0.704 with relative diagnostic specificity and sensitivity of 96.6% and 94.5%, respectively. These findings suggest that the developed rgB-iELISA has strong potential for large-scale IBR serosurveillance following further validation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of recombinant glycoprotein B based Indirect ELISA for detection of antibodies against Bovine alphaherpesvirus-1 in Bovines

  • 期刊论文

  • 发表日期：

    2026年6月26日

查看全文
  • K. S. Lakshmi,
  • Sharanagouda S. Patil,
  • Siju Susan Jacob,
  • A. R. Chandni,
  • J. Manjunatha,
  • R. Sridevi

摘要

Infectious Bovine Rhinotracheitis (IBR) is a highly contagious respiratory disease of cattle caused by Bovine alphaherpesvirus-1 (BoAHV-1), a member of the genus Varicellovirus. Similar to other alphaherpesviruses, BoAHV-1 establishes lifelong latency with the potential for reactivation under immunosuppressive or stressful conditions. The viral envelope contains several glycoproteins, among which gB, gC, and gD are the major immunogens recognized by sera of infected cattle. Glycoprotein B (gB) is highly conserved and strongly immunogenic, making it a suitable target for diagnostic assay development. In this study, a truncated gB fragment was cloned and expressed in Escherichia coli. The recombinant gB (rgB) (~ 47 kDa) was purified under denaturing conditions using Ni-NTA affinity chromatography and confirmed by SDS-PAGE and western blotting. The protein showed strong reactivity with anti-His antibodies and IBR-positive bovine sera, indicating preserved immunoreactive epitopes. The purified protein was standardized as a coating antigen in an indirect ELISA (iELISA). Diagnostic evaluation using 162 sera, compared with the ID Screen® IBR gB Competition ELISA, yielded a ROC-determined cut-off OD of 0.704 with relative diagnostic specificity and sensitivity of 96.6% and 94.5%, respectively. These findings suggest that the developed rgB-iELISA has strong potential for large-scale IBR serosurveillance following further validation.