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<p>The worldwide climate is thought to be drastically changing as a result of the global temperatures, a phenomenon known as “global warming”. Thermal stress is a crucial obstacle facing buffalo cyclicity. Investigation of the molecular regulation concerning proliferation and apoptosis of corpus luteum (CL) is not fully comprehended in buffaloes. We aimed to (1) study mRNA expression of candidate genes related to proliferation (PGR, AGTR1, and LHCGR) and apoptosis (TNF<sub>α</sub>, BAX, FASLG, CASP3, AGTR2 and PTGS2), (2) explore effect of thermal stress on the expression of HSP70, NOS1, NOS2 mRNAs, NO and SOD concentrations in CL homogenate during different stages of CL. For this, ovaries (<i>n</i> = 70) were collected in pairs from buffaloes during cold and hot seasons. According to morphology of CL, samples were divided into: early, mid, and late. For RNA isolation, NO and SOD concentrations, small sections from CL stages were frozen in − 80&#xa0;°C. The results showed that PGR, AGTR2, TNF<sub>α</sub>, BAX, cALP2beta and PTGS2 mRNAs decreased (<i>P</i> &lt; 0.001) at different stages of CL at hot season. The decline of AGTR2 associated with decreased NOS2 mRNA, which consequently affected TNF<sub>α</sub>, BAX, and CASP3 mRNAs. Apoptosis might be affected by direct effect of AGTR2 on CASP3 during thermal stress. We supposed that NO had a regulatory role during early and late stages of CL. It could be concluded that thermal stress (THI &gt; 68) changed the expression of proliferation and apoptosis genes of CL in Egyptian buffaloes. Finally, the thermal stress in cold or hot seasons has marked impact on CL dynamics.</p>

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Relative abundance of genes regulating proliferation and apoptosis of corpus luteum during cold and hot seasons in Egyptian buffaloes

  • 期刊论文

  • 发表日期：

    2026年6月22日

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  • Samaa M. Galal,
  • Sally Ibrahim,
  • Karima Mahmoud,
  • Ola Adel,
  • Aya A. Shokry,
  • M. S. El-Belely,
  • S. T. Ismail

摘要

The worldwide climate is thought to be drastically changing as a result of the global temperatures, a phenomenon known as “global warming”. Thermal stress is a crucial obstacle facing buffalo cyclicity. Investigation of the molecular regulation concerning proliferation and apoptosis of corpus luteum (CL) is not fully comprehended in buffaloes. We aimed to (1) study mRNA expression of candidate genes related to proliferation (PGR, AGTR1, and LHCGR) and apoptosis (TNFα, BAX, FASLG, CASP3, AGTR2 and PTGS2), (2) explore effect of thermal stress on the expression of HSP70, NOS1, NOS2 mRNAs, NO and SOD concentrations in CL homogenate during different stages of CL. For this, ovaries (n = 70) were collected in pairs from buffaloes during cold and hot seasons. According to morphology of CL, samples were divided into: early, mid, and late. For RNA isolation, NO and SOD concentrations, small sections from CL stages were frozen in − 80 °C. The results showed that PGR, AGTR2, TNFα, BAX, cALP2beta and PTGS2 mRNAs decreased (P < 0.001) at different stages of CL at hot season. The decline of AGTR2 associated with decreased NOS2 mRNA, which consequently affected TNFα, BAX, and CASP3 mRNAs. Apoptosis might be affected by direct effect of AGTR2 on CASP3 during thermal stress. We supposed that NO had a regulatory role during early and late stages of CL. It could be concluded that thermal stress (THI > 68) changed the expression of proliferation and apoptosis genes of CL in Egyptian buffaloes. Finally, the thermal stress in cold or hot seasons has marked impact on CL dynamics.