Scholarlink

<p>This study investigated the effects of ruminal tannase-producing bacteria (TPB) on growth performance, nutrient digestibility, blood parameters, and rumen bacterial populations in weaning Afshar–Kurdi lambs fed pomegranate peel (PP). Forty-nine strains representing the genera <i>Klebsiella</i>, <i>Escherichia</i>, <i>Raoultella</i>, <i>Enterobacter</i>, and <i>Pectobacterium</i>, used to formulate an oral feed additive (OFA). The OFA was administered to thirty-six lambs aged 1–70 days, which were randomly assigned to four treatments: T1 (basal diet), T2 (basal diet + 1 mL OFA), T3 (basal diet + 5% PP), and T4 (basal diet + 1 mL OFA + 5% PP). Growth performance was not affected by dietary treatments, as dry matter intake (DMI), body weight (BW), and average daily gain (ADG) remained unchanged (<i>P</i> &gt; 0.05). However, birth weight significantly influenced DMI, BW, and ADG (<i>P</i> &lt; 0.05). OFA reduced ruminal pH (<i>P</i> &lt; 0.0001) and improved digestibility of fiber fractions, particularly ADF and cellulose (<i>P</i> &lt; 0.01). In contrast, PP increased ruminal pH and NH₃–N concentration (<i>P</i> = 0.009) and decreased DM and CP digestibility (<i>P</i> &lt; 0.05). Significant OFA × PP interactions were observed for DM, CP, CF, NDF, and ADF digestibility (<i>P</i> ≤ 0.01), indicating interactive effects on fiber utilization. Blood metabolites were largely unaffected; however, OFA reduced serum uric acid (UA)&#xa0;by 60.5% (0.58 vs. 1.47&#xa0;mg/dL; <i>P</i> = 0.01) and increased albumin (ALB)&#xa0;and Glutamate oxaloacetate transaminase (GOT, <i>P</i> &lt; 0.05, while creatinine (CRE)&#xa0;remained unchanged. DGGE analysis revealed treatment-dependent shifts in ruminal bacterial communities, with OFA-supplemented groups showing greater band richness (≈ 11–14 bands per lane) than control and PP-only groups (&lt; 7 bands). Overall, OFA, particularly when combined with PP, enhanced ruminal microbial diversity and fiber digestion without affecting growth performance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ruminal tannase-producing bacteria and their effects on growth performance, nutrient digestibility, blood parameters, and rumen bacterial populations in weaning Afshar-Kurdi lambs fed pomegranate peel

  • 期刊论文

  • 发表日期：

    2026年5月28日

查看全文
  • Maryam Gheibipour,
  • Seyyed Ehsan Ghiasi,
  • Mohammad Bagher Montazer Torbati,
  • Moslem Bashtani,
  • Hossein Motamedi,
  • Mudasir Ahmad Dar

摘要

This study investigated the effects of ruminal tannase-producing bacteria (TPB) on growth performance, nutrient digestibility, blood parameters, and rumen bacterial populations in weaning Afshar–Kurdi lambs fed pomegranate peel (PP). Forty-nine strains representing the genera Klebsiella, Escherichia, Raoultella, Enterobacter, and Pectobacterium, used to formulate an oral feed additive (OFA). The OFA was administered to thirty-six lambs aged 1–70 days, which were randomly assigned to four treatments: T1 (basal diet), T2 (basal diet + 1 mL OFA), T3 (basal diet + 5% PP), and T4 (basal diet + 1 mL OFA + 5% PP). Growth performance was not affected by dietary treatments, as dry matter intake (DMI), body weight (BW), and average daily gain (ADG) remained unchanged (P > 0.05). However, birth weight significantly influenced DMI, BW, and ADG (P < 0.05). OFA reduced ruminal pH (P < 0.0001) and improved digestibility of fiber fractions, particularly ADF and cellulose (P < 0.01). In contrast, PP increased ruminal pH and NH₃–N concentration (P = 0.009) and decreased DM and CP digestibility (P < 0.05). Significant OFA × PP interactions were observed for DM, CP, CF, NDF, and ADF digestibility (P ≤ 0.01), indicating interactive effects on fiber utilization. Blood metabolites were largely unaffected; however, OFA reduced serum uric acid (UA) by 60.5% (0.58 vs. 1.47 mg/dL; P = 0.01) and increased albumin (ALB) and Glutamate oxaloacetate transaminase (GOT, P < 0.05, while creatinine (CRE) remained unchanged. DGGE analysis revealed treatment-dependent shifts in ruminal bacterial communities, with OFA-supplemented groups showing greater band richness (≈ 11–14 bands per lane) than control and PP-only groups (< 7 bands). Overall, OFA, particularly when combined with PP, enhanced ruminal microbial diversity and fiber digestion without affecting growth performance.