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<p>High-Resolution Melting (HRM) analysis is gaining prominence as a powerful molecular diagnostic tool in veterinary medicine, offering rapid and cost-effective detection of pathogens and genetic variants. This systematic review synthesizes findings from 52 studies, underscoring the utility of HRM in the early identification of infectious agents in livestock. The review also examines its effectiveness in differentiating closely related pathogens and in monitoring antimicrobial resistance. These applications directly contribute to more informed treatment decisions, enhanced disease surveillance, and improved biosecurity strategies in both companion and production animals. A targeted meta-analysis of six studies encompassing 692 clinical samples revealed high diagnostic accuracy, with pooled sensitivity of 94.3% and specificity of 98.3%. These results affirm HRM’s reliability as a frontline screening tool, particularly valuable during disease outbreaks or herd health assessments where speed and precision are critical. Despite its advantages, HRM has certain limitations. It depends on high-quality DNA, may overestimate low-abundance targets, and often struggles to distinguish between highly related genotypes. Nevertheless, ongoing innovations, including multiplex HRM assay development, continue to enhance its applicability to a wider array of veterinary pathogens. As the field evolves, integrating HRM into routine diagnostic workflows may empower veterinarians to make faster and more accurate decisions, ultimately promoting better animal health outcomes.</p>

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High-resolution melting analysis in veterinary medicine: a systematic review of applications and diagnostic accuracy

  • 期刊论文

  • 发表日期：

    2025年10月29日

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  • Oluwagbemiga Ademola Dada,
  • Lais Goulart Ribeiro,
  • Natalia Machado Rahal,
  • Kauê Rodriguez Martins,
  • Rodrigo Casquero Cunha,
  • Odir Antonio Dellagostin

摘要

High-Resolution Melting (HRM) analysis is gaining prominence as a powerful molecular diagnostic tool in veterinary medicine, offering rapid and cost-effective detection of pathogens and genetic variants. This systematic review synthesizes findings from 52 studies, underscoring the utility of HRM in the early identification of infectious agents in livestock. The review also examines its effectiveness in differentiating closely related pathogens and in monitoring antimicrobial resistance. These applications directly contribute to more informed treatment decisions, enhanced disease surveillance, and improved biosecurity strategies in both companion and production animals. A targeted meta-analysis of six studies encompassing 692 clinical samples revealed high diagnostic accuracy, with pooled sensitivity of 94.3% and specificity of 98.3%. These results affirm HRM’s reliability as a frontline screening tool, particularly valuable during disease outbreaks or herd health assessments where speed and precision are critical. Despite its advantages, HRM has certain limitations. It depends on high-quality DNA, may overestimate low-abundance targets, and often struggles to distinguish between highly related genotypes. Nevertheless, ongoing innovations, including multiplex HRM assay development, continue to enhance its applicability to a wider array of veterinary pathogens. As the field evolves, integrating HRM into routine diagnostic workflows may empower veterinarians to make faster and more accurate decisions, ultimately promoting better animal health outcomes.