Scholarlink

<p>Theileriosis is a significant protozoan disease in cattle, transmitted by ticks, and poses a major health concern for bovine populations. With rising case numbers and strain-specific clinical variability, the genetic characterization of <i>Theileria</i> species in different geographical regions has become crucial. However, limited molecular data are available on the genetic diversity and identity matrix analysis of <i>Theileria</i> species in cattle. This study aimed to genetically evaluate <i>Theileria</i> species isolates affecting cattle in the Western parts of Uttar Pradesh, India. In the present investigation, one hundred blood samples were collected from bovines exhibiting clinical signs consistent with Theileria infection in the study. Initial screening was conducted using Giemsa-stained thin blood smears, followed by molecular validation confirmation using polymerase chain reaction (PCR) targeting the 18&#xa0;S ribosomal RNA (rRNA) gene. Genetic analyses were performed using the MEGA-X software. Microscopic examination identified <i>Theileria</i> species in 17% of the samples, whereas PCR detected the parasite in 53% of the examined samples. Following 18&#xa0;S rRNA gene sequencing and bioinformatic analysis, all field isolates were found to be clustered into a single clade, showing 95.96–98.49% sequence homology with <i>Theileria annulata</i>. These findings highlight the significance of improving molecular surveillance and genetic profiling of <i>Theileria</i> species across diverse regions to better understand their epidemiology and inform control strategies for bovine theileriosis in India.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular characterization and phylogenetic insights into Theileria annulata affecting cattle in Western Uttar Pradesh, India

  • 期刊论文

  • 发表日期：

    2025年10月7日

查看全文
  • Vishnu Kumar Rai,
  • Arbind Singh,
  • Amit Kumar Verma,
  • Prem Sagar Maurya,
  • Vikas Jaiswal,
  • Ramakant,
  • Surendra Upadhyay,
  • Ayush Pal

摘要

Theileriosis is a significant protozoan disease in cattle, transmitted by ticks, and poses a major health concern for bovine populations. With rising case numbers and strain-specific clinical variability, the genetic characterization of Theileria species in different geographical regions has become crucial. However, limited molecular data are available on the genetic diversity and identity matrix analysis of Theileria species in cattle. This study aimed to genetically evaluate Theileria species isolates affecting cattle in the Western parts of Uttar Pradesh, India. In the present investigation, one hundred blood samples were collected from bovines exhibiting clinical signs consistent with Theileria infection in the study. Initial screening was conducted using Giemsa-stained thin blood smears, followed by molecular validation confirmation using polymerase chain reaction (PCR) targeting the 18 S ribosomal RNA (rRNA) gene. Genetic analyses were performed using the MEGA-X software. Microscopic examination identified Theileria species in 17% of the samples, whereas PCR detected the parasite in 53% of the examined samples. Following 18 S rRNA gene sequencing and bioinformatic analysis, all field isolates were found to be clustered into a single clade, showing 95.96–98.49% sequence homology with Theileria annulata. These findings highlight the significance of improving molecular surveillance and genetic profiling of Theileria species across diverse regions to better understand their epidemiology and inform control strategies for bovine theileriosis in India.