In vitro gas production, in situ digestibility, intake, weight gain and ruminal characteristics of calves fed a diet containing 60% waste papaya silage

Our aim was to evaluate a whole-grain diet containing 0 (T1) and 60% (T2) waste papaya silage in vitro, in situ and in vivo. In vitro, biogas, methane and dry matter degradation (DMDiv), neutral detergent fiber (NDFDiv) and crude protein (CPDiv) were determined at 72 h; in situ, DM (DMDis), NDF (NDFis) and CP (CPDis) digestibility was determined at 72 h; in vivo, consumption and apparent digestibility of DM (DMI and DMD) and NDF (NDFI and NDFD), daily weight gain (DWG), rumen characteristics (pH, bacterial and protozoan counts) were determined. In addition, we performed coproparasitoscopic analysis and interviews with producers. Biogas production, methane, DMDiv, CDPiv, DMDis, DMD, NDFD, pH, bacterial and protozoan counts, and parasite load of nematodes and coccidia were not different between treatments (p > 0.05). T2 showed more NDFDiv, CPDis, DMI, NDFI, DWG than T1, while T1 was higher than T2 in NDFDis (p < 0.05). The results obtained from in vitro, in situ and in vivo techniques indicate that the diet with 60% waste papaya silage (T2) showed a similar response to the control diet (T1). Therefore, waste papaya silage is a non-conventional feeding alternative for weaned calves in the Costa Chica region of the state of Guerrero, Mexico.