<p>Low temperature is a common abiotic stress, which can seriously restrict the normal growth and development of crops. In this study, wheat cold-regulated gene 80 (<i>Wcor80</i>) was isolated from “Dongnongdongmai 1” (Dn1) material and characterized. The expression of <i>Wcor80</i> was induced by brassinolide (BR) and low temperature (LT) treatments, and it was preferentially expressed in the leaves under abscisic acid (ABA) treatment and in the tillering nodes under BR treatment. Overexpression of <i>Wcor80</i> in transgenic <i>Arabidopsis</i> significantly increased tolerance to LT treatment. Overall, our findings suggest that <i>Wcor80</i> functions as a positive regulator of resistance to LT stress, providing a theoretical basis for the molecular breeding and cultivation regulation of winter wheat.</p>

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Overexpression of wheat Wcor80 confers tolerance to low temperature in transgenic plant

  • Luyao Wang,
  • Jiaang Cao,
  • Xuesong Wang,
  • Junbao Zhang,
  • Yushu Chen,
  • Sen Yang,
  • Qingyi Shao,
  • Mengdi Yu,
  • Zhongmin Jin,
  • Lijie Liu

摘要

Low temperature is a common abiotic stress, which can seriously restrict the normal growth and development of crops. In this study, wheat cold-regulated gene 80 (Wcor80) was isolated from “Dongnongdongmai 1” (Dn1) material and characterized. The expression of Wcor80 was induced by brassinolide (BR) and low temperature (LT) treatments, and it was preferentially expressed in the leaves under abscisic acid (ABA) treatment and in the tillering nodes under BR treatment. Overexpression of Wcor80 in transgenic Arabidopsis significantly increased tolerance to LT treatment. Overall, our findings suggest that Wcor80 functions as a positive regulator of resistance to LT stress, providing a theoretical basis for the molecular breeding and cultivation regulation of winter wheat.