<p>Sweet potatoes (<i>Ipomoea batatas</i> L.) have agronomic and nutritional importance, being cultivated in tropical and subtropical regions due to their hardiness. Its traditional method of propagation, through vegetative cuttings, favors the spread of pathogens. Biotechnological techniques, such as micropropagation, are effective in obtaining healthy and uniform seedlings. Micropropagation is carried out in a fully controlled environment, which makes it possible to evaluate the effect of physical stimuli, such as sound, on the plants. Sound, through acoustic vibrations, can modulate physiological and structural processes in plants. In this study, we evaluated the effects of sound stimuli (rock music, classical, and bird sounds) and their absence (control) on biometric characteristics, photosynthetic pigments, and morphological aspects of sweet potato plants grown in vitro. The experiment was a completely randomized design, with 14 replicates per treatment. Nodal segments were cultured in MS medium and exposed to sound stimuli at 90 dB for 2&#xa0;h daily for 30 days. Rock music as a sound stimulus resulted in greater plant height, number of roots, nodes, and senescent leaves, as well as greater fresh and dry root mass, while leaf area was reduced. Chlorophyll and carotenoid levels were higher in plants exposed to sound stimuli than in their absence. Morphological analyses revealed epidermal alterations under the rock music, with a lower apparent density of trichomes, which were larger and had stomata presence. The epidermal surface of these plants was rougher. It is concluded that sound stimuli can influence the biometry and morphology of sweet potato plants, with rock music causing the greatest number of alterations when compared to the absence of sound.</p>

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Sound stimuli alter biometric characteristics, photosynthetic pigments, and morphology of sweet potato plants in vitro

  • Evilane do Nascimento Monteiro,
  • Marcelo de Almeida Guimarães,
  • Gabrielen de Maria Gomes Dias,
  • Caris dos Santos Viana

摘要

Sweet potatoes (Ipomoea batatas L.) have agronomic and nutritional importance, being cultivated in tropical and subtropical regions due to their hardiness. Its traditional method of propagation, through vegetative cuttings, favors the spread of pathogens. Biotechnological techniques, such as micropropagation, are effective in obtaining healthy and uniform seedlings. Micropropagation is carried out in a fully controlled environment, which makes it possible to evaluate the effect of physical stimuli, such as sound, on the plants. Sound, through acoustic vibrations, can modulate physiological and structural processes in plants. In this study, we evaluated the effects of sound stimuli (rock music, classical, and bird sounds) and their absence (control) on biometric characteristics, photosynthetic pigments, and morphological aspects of sweet potato plants grown in vitro. The experiment was a completely randomized design, with 14 replicates per treatment. Nodal segments were cultured in MS medium and exposed to sound stimuli at 90 dB for 2 h daily for 30 days. Rock music as a sound stimulus resulted in greater plant height, number of roots, nodes, and senescent leaves, as well as greater fresh and dry root mass, while leaf area was reduced. Chlorophyll and carotenoid levels were higher in plants exposed to sound stimuli than in their absence. Morphological analyses revealed epidermal alterations under the rock music, with a lower apparent density of trichomes, which were larger and had stomata presence. The epidermal surface of these plants was rougher. It is concluded that sound stimuli can influence the biometry and morphology of sweet potato plants, with rock music causing the greatest number of alterations when compared to the absence of sound.