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<p>In this study, the effect of gamma-irradiated pollen on the frequency of haploid embryos and regenerants produced from unfertilized ovaries by application of polyamines (putrescine and spermidine) and cycocel (CCC) in female cucumber genotypes (“Storm”, “Sahm”, and “547”) was evaluated. Female plants were sprayed from12 days before until 12 days after anthesis with 1-day intervals, with a mixture of putrescine (500 mg L<sup>− 1</sup>), spermidine (50 mg L<sup>− 1</sup>), and CCC (50 mg L<sup>− 1</sup>). Additionally, gamma irradiation at doses of 400, 500, and 600 Gy was used to sterilize pollen grains. The rate of embryo induction and regenerated plantlets varied across irradiation doses depending on the genotype. The highest number of embryos (6) and plantlets (5) were obtained from the “Storm” genotype at 600 Gy; no embryos were observed in the control. The highest embryogenesis frequency (5.68%) and regeneration (93.88%) was observed in the “547” and “Sahm” genotypes at 500&#xa0;Gy, respectively. Ploidy analysis using flow cytometry revealed that all regenerated plantlets contained the gametic chromosome number (n = x = 7) of the parental plants, and SSR analysis confirmed homozygosity in the doubled haploid (DH) lines. The findings indicated that optimized pollen irradiation, appropriate genotypes and application of polyamines can serve as effective steps toward the production of doubled haploids in cucumber hybrid breeding programs.</p>

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Improvement of pollen-induced haploid parthenogenesis by polyamine and cycocel treatments in cucumber (Cucumis Sativus L.)

  • 期刊论文

  • 发表日期：

    2026年7月9日

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  • Sakineh Farhadi-Tooli,
  • Mehran E. Shariatpanahi,
  • Mohammadvali Habibi-Silabi,
  • Maryam Tavakoli,
  • Zahra Sadaat Hosseini

摘要

In this study, the effect of gamma-irradiated pollen on the frequency of haploid embryos and regenerants produced from unfertilized ovaries by application of polyamines (putrescine and spermidine) and cycocel (CCC) in female cucumber genotypes (“Storm”, “Sahm”, and “547”) was evaluated. Female plants were sprayed from12 days before until 12 days after anthesis with 1-day intervals, with a mixture of putrescine (500 mg L− 1), spermidine (50 mg L− 1), and CCC (50 mg L− 1). Additionally, gamma irradiation at doses of 400, 500, and 600 Gy was used to sterilize pollen grains. The rate of embryo induction and regenerated plantlets varied across irradiation doses depending on the genotype. The highest number of embryos (6) and plantlets (5) were obtained from the “Storm” genotype at 600 Gy; no embryos were observed in the control. The highest embryogenesis frequency (5.68%) and regeneration (93.88%) was observed in the “547” and “Sahm” genotypes at 500 Gy, respectively. Ploidy analysis using flow cytometry revealed that all regenerated plantlets contained the gametic chromosome number (n = x = 7) of the parental plants, and SSR analysis confirmed homozygosity in the doubled haploid (DH) lines. The findings indicated that optimized pollen irradiation, appropriate genotypes and application of polyamines can serve as effective steps toward the production of doubled haploids in cucumber hybrid breeding programs.