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<p><i>Emilia sonchifolia</i> (L.) DC. is an annual herbaceous plant of the Asteraceae, well known for its various health benefits and pharmaceutically important biochemical constituents. It is also used in Indian traditional systems like Unani and Ayurveda, Chinese and Nigerian traditional systems. The aim of current study to establish an efficient in vitro propagation protocol and evaluate the phytochemical and biological potential of callus derived extracts. Leaf explants revealed the highest callus induction (95.20%) on MS medium with 2,4-D (1.5&#xa0;mg/L) and Kn (2.0&#xa0;mg/L). Highest shoot regeneration (85.96%; 45.40 shoots/explant) was observed with Kn (1.0&#xa0;mg/L) and BAP (1.0&#xa0;mg/L), while nodal explants showed efficient direct organogenesis on Kn (1.0&#xa0;mg/L). MS medium containing NAA (1.0&#xa0;mg/L) exhibits the highest rooting (96.00%) and acclimatized plants showed a survival rate of 90%. Phytochemical analysis showed significantly higher phenolic, flavonoid and tannin contents in ethanolic callus extract compared to mother plant. GC-MS analysis revealed 48 chemical constituents, including ethyl esters, fatty acids, triterpenes, phenolics and phytosterols. Additionally, ethanolic callus extract exhibited antioxidant (DPPH and ABTS), antidiabetic (α-amylase and α-glucosidase inhibition), and cytotoxic activities (HepG2 and PANC-1 cell lines) than the mother plant. The study highlights that in vitro cultures of <i>E. sonchifolia</i> may serves as an efficient in vitro protocol for large scale propagation and potential source of bioactive constituents for further investigation.</p>

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In vitro regeneration and enhanced bioactive potential of callus cultures in Emilia sonchifolia (L.) DC.

  • 期刊论文

  • 发表日期：

    2026年7月7日

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  • Mahantesh Banni,
  • M. Jayaraj

摘要

Emilia sonchifolia (L.) DC. is an annual herbaceous plant of the Asteraceae, well known for its various health benefits and pharmaceutically important biochemical constituents. It is also used in Indian traditional systems like Unani and Ayurveda, Chinese and Nigerian traditional systems. The aim of current study to establish an efficient in vitro propagation protocol and evaluate the phytochemical and biological potential of callus derived extracts. Leaf explants revealed the highest callus induction (95.20%) on MS medium with 2,4-D (1.5 mg/L) and Kn (2.0 mg/L). Highest shoot regeneration (85.96%; 45.40 shoots/explant) was observed with Kn (1.0 mg/L) and BAP (1.0 mg/L), while nodal explants showed efficient direct organogenesis on Kn (1.0 mg/L). MS medium containing NAA (1.0 mg/L) exhibits the highest rooting (96.00%) and acclimatized plants showed a survival rate of 90%. Phytochemical analysis showed significantly higher phenolic, flavonoid and tannin contents in ethanolic callus extract compared to mother plant. GC-MS analysis revealed 48 chemical constituents, including ethyl esters, fatty acids, triterpenes, phenolics and phytosterols. Additionally, ethanolic callus extract exhibited antioxidant (DPPH and ABTS), antidiabetic (α-amylase and α-glucosidase inhibition), and cytotoxic activities (HepG2 and PANC-1 cell lines) than the mother plant. The study highlights that in vitro cultures of E. sonchifolia may serves as an efficient in vitro protocol for large scale propagation and potential source of bioactive constituents for further investigation.