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<p>Production of genetically stable inbred carrot (<i>Daucus carota</i> L.) lines is a challenging task due to the protandrous nature of carrot. This important biennial cool season root crop can benefit from androgenesis-based gametic embryogenesis technique whereby embryos and plants develop from immature male gametes. This process allows for the production of plants with half the chromosome number (haploid). Subsequent spontaneous or induced chromosome doubling results in development of doubled haploid (DH) plants. When applied successfully, the androgenesis technique may allow production of homozygous lines invaluable in carrot breeding programs. This study was carried out to investigate the androgenic plant production potential of five orange- and five purple-rooted carrot genotypes via anther culture. A total of 11,640 anthers containing late uni-nucleate and early bi-nucleate stage microspores were cultured in modified B5 medium. Responsive anthers were detected about three months after culture initiation. Androgenesis response was detected in 4.78% of the cultured carrot anthers providing 6.14 androgenic plantlets per responding anther. A total of 3420 androgenic plantlets were obtained, and 2020 of them (59.06%) were grown into plants. Carrot genotypes investigated in this study showed substantial differences in their responses to androgenesis induction. Androgenic plantlets and plants were obtained from four orange-rooted and five purple-rooted genotypes. Flow cytometry analysis revealed that 1.84% of the androgenic carrot plants were haploid, while 94.85% and 3.31% were diploid and tetraploid, respectively. The majority of the DH plants were successfully acclimatized, grown in an unheated greenhouse and self-pollinated for the seed production. One hundred and forty-six flowering DH plants produced seeds at yield levels ranging from low (1–300) to high (&gt;1000). The results obtained from this study clearly show that the androgenesis technique can provide large numbers of fertile DH carrot plants from Turkish orange- and purple-rooted genotypes.</p>

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Doubled haploid orange and purple rooted plants derived from Turkish carrot (Daucus carota L.) germplasm via anther culture

  • 期刊论文

  • 发表日期：

    2026年7月7日

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  • Alireza Lachin,
  • Ali Ramazan Alan

摘要

Production of genetically stable inbred carrot (Daucus carota L.) lines is a challenging task due to the protandrous nature of carrot. This important biennial cool season root crop can benefit from androgenesis-based gametic embryogenesis technique whereby embryos and plants develop from immature male gametes. This process allows for the production of plants with half the chromosome number (haploid). Subsequent spontaneous or induced chromosome doubling results in development of doubled haploid (DH) plants. When applied successfully, the androgenesis technique may allow production of homozygous lines invaluable in carrot breeding programs. This study was carried out to investigate the androgenic plant production potential of five orange- and five purple-rooted carrot genotypes via anther culture. A total of 11,640 anthers containing late uni-nucleate and early bi-nucleate stage microspores were cultured in modified B5 medium. Responsive anthers were detected about three months after culture initiation. Androgenesis response was detected in 4.78% of the cultured carrot anthers providing 6.14 androgenic plantlets per responding anther. A total of 3420 androgenic plantlets were obtained, and 2020 of them (59.06%) were grown into plants. Carrot genotypes investigated in this study showed substantial differences in their responses to androgenesis induction. Androgenic plantlets and plants were obtained from four orange-rooted and five purple-rooted genotypes. Flow cytometry analysis revealed that 1.84% of the androgenic carrot plants were haploid, while 94.85% and 3.31% were diploid and tetraploid, respectively. The majority of the DH plants were successfully acclimatized, grown in an unheated greenhouse and self-pollinated for the seed production. One hundred and forty-six flowering DH plants produced seeds at yield levels ranging from low (1–300) to high (>1000). The results obtained from this study clearly show that the androgenesis technique can provide large numbers of fertile DH carrot plants from Turkish orange- and purple-rooted genotypes.