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<p><i>Valeriana jatamansi</i> is an important medicinal herb grows naturally at high altitude in the temperate region of Himalayas that requires conservation. The species contain valuable phytochemicals including valerenic acid, volatile oils, iridoids, valepotriates and sesquiterpenoids which shows a diverse range of biological activities. Developing an effective regeneration protocol is necessary for mass propagation and germplasm conservation for the sustainable utilization of this high value Himalayan medicinal plant. The current study aimed to develop brassinolide (BR)-mediated regeneration system of <i>V. jatamansi</i> using nodal explants. The highest number of shoots (2.0 ± 0.58) and number of branches (11.67 ± 2.03) was produced after eight weeks of time in the control when compared to the different concentrations of brassinolide. The maximum shoot length (10.67 ± 0.33&#xa0;cm), number of roots (39.0 ± 2.89), number of leaves (13.0 ± 1.0) and fresh biomass (6.25 ± 0.67 gm) and dry biomass (2.01 ± 0.84 gm) was recorded on MS medium supplemented with BR (0.03&#xa0;mg/L). The genetic uniformity of in vitro raised plantlets and mother plant of <i>V. jatamansi</i> was verified by using start codon targeted (SCoT) marker, revealed a monomorphic banding pattern across all analyzed regenerants, indicating high genetic uniformity among the tested in vitro raised plantlets. Thus, the established method may facilitate promising efforts in <i>ex situ</i> conservation of medicinally important Himalayan plant <i>V. jatamansi</i> and also develop a foundation for future research related to metabolic engineering and other biotechnological applications.</p>

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Brassinolide (BR)-mediated in vitro regeneration and genetic stability assessment of Valeriana jatamansi: an important Himalayan medicinal plant

  • 期刊论文

  • 发表日期：

    2026年6月29日

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  • Grace Gudu Joseph,
  • Saikat Sena,
  • Vijay Kumar

摘要

Valeriana jatamansi is an important medicinal herb grows naturally at high altitude in the temperate region of Himalayas that requires conservation. The species contain valuable phytochemicals including valerenic acid, volatile oils, iridoids, valepotriates and sesquiterpenoids which shows a diverse range of biological activities. Developing an effective regeneration protocol is necessary for mass propagation and germplasm conservation for the sustainable utilization of this high value Himalayan medicinal plant. The current study aimed to develop brassinolide (BR)-mediated regeneration system of V. jatamansi using nodal explants. The highest number of shoots (2.0 ± 0.58) and number of branches (11.67 ± 2.03) was produced after eight weeks of time in the control when compared to the different concentrations of brassinolide. The maximum shoot length (10.67 ± 0.33 cm), number of roots (39.0 ± 2.89), number of leaves (13.0 ± 1.0) and fresh biomass (6.25 ± 0.67 gm) and dry biomass (2.01 ± 0.84 gm) was recorded on MS medium supplemented with BR (0.03 mg/L). The genetic uniformity of in vitro raised plantlets and mother plant of V. jatamansi was verified by using start codon targeted (SCoT) marker, revealed a monomorphic banding pattern across all analyzed regenerants, indicating high genetic uniformity among the tested in vitro raised plantlets. Thus, the established method may facilitate promising efforts in ex situ conservation of medicinally important Himalayan plant V. jatamansi and also develop a foundation for future research related to metabolic engineering and other biotechnological applications.