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<p><i>Zataria multiflora</i> Boiss, commonly known as Shirazi thyme, is a prominent medicinal plant widely cultivated in Iran due to its high demand for significant applications across the pharmaceutical, food and cosmetics industries. In the present study, various concentrations of methyl jasmonate (MeJA) and titanium dioxide nanoparticles (TiO<sub>2</sub>NPs) were systematically evaluated to optimize thymol production in <i>Z. multiflora</i> suspension cell cultures. First, the leaf and stem explants were cultured in MS and B5 basal media supplemented with 2,4-D (0 or 2&#xa0;mg L⁻¹), kin (0 or 1&#xa0;mg L⁻¹), BAP (0, 0.5, 1, or 1.5&#xa0;mg L⁻¹), or NAA (0, 0.5, 1, or 1.5&#xa0;mg L⁻¹). Calli were subsequently transferred to suspension culture media and treated with MeJA and TiO<sub>2</sub>NPs to assess their effects on thymol production. Optimal callus induction (100%) and callus fresh weight (5&#xa0;g) were achieved by culturing leaf explants on B5 media supplemented with 2&#xa0;mg L⁻¹ 2,4-D, 1&#xa0;mg L⁻¹ BAP, and 1&#xa0;mg L⁻¹ kin. Used HPLC to show that highest thymol content in the suspension culture (14&#xa0;mg/g dry weight) was observed with the application of 22.43&#xa0;mg L⁻¹ MeJA and 50&#xa0;mg L⁻¹ TiO₂NPs. RT‒PCR confirmed <i>TPS2</i> gene expression in elicitor-treated suspension cultures.</p>

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The effect of titanium dioxide nanoparticles (TiO₂ NPs) and methyl jasmonate (MeJA) on thymol content and terpinen synthase-ϒ (TPS-ϒ) gene expression in suspension cultures of Zataria multiflora Boiss

  • 期刊论文

  • 发表日期：

    2026年7月7日

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  • Sahra Rashidi Bajgani,
  • Nasim zarinpanjeh,
  • Amrollah Nabigol

摘要

Zataria multiflora Boiss, commonly known as Shirazi thyme, is a prominent medicinal plant widely cultivated in Iran due to its high demand for significant applications across the pharmaceutical, food and cosmetics industries. In the present study, various concentrations of methyl jasmonate (MeJA) and titanium dioxide nanoparticles (TiO2NPs) were systematically evaluated to optimize thymol production in Z. multiflora suspension cell cultures. First, the leaf and stem explants were cultured in MS and B5 basal media supplemented with 2,4-D (0 or 2 mg L⁻¹), kin (0 or 1 mg L⁻¹), BAP (0, 0.5, 1, or 1.5 mg L⁻¹), or NAA (0, 0.5, 1, or 1.5 mg L⁻¹). Calli were subsequently transferred to suspension culture media and treated with MeJA and TiO2NPs to assess their effects on thymol production. Optimal callus induction (100%) and callus fresh weight (5 g) were achieved by culturing leaf explants on B5 media supplemented with 2 mg L⁻¹ 2,4-D, 1 mg L⁻¹ BAP, and 1 mg L⁻¹ kin. Used HPLC to show that highest thymol content in the suspension culture (14 mg/g dry weight) was observed with the application of 22.43 mg L⁻¹ MeJA and 50 mg L⁻¹ TiO₂NPs. RT‒PCR confirmed TPS2 gene expression in elicitor-treated suspension cultures.