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<p><i>Melastoma normale</i> D. Don is a multifunctional shrub in the genus <i>Melastoma</i> and is highly ornamental. This study establishes an efficient in vitro regeneration system for <i>M. normale</i> and identifies key factors influencing adventitious bud induction. Leaf segments demonstrated exclusive regenerative capacity, with an 84.67% shoot induction rate, whereas petiole and stem explants were unsuitable. The orientation of explants significantly affected shoot regeneration efficiency, with the abaxial side facing the medium yielding superior results. Among basal media, 1/2MS supported optimal regeneration and explant vitality. Plant growth regulators (PGR) combinations critically determined the regeneration pathway. Shoot formation was achieved specifically with 2&#xa0;mg/L BA and 0.2&#xa0;mg/L NAA. In contrast, combinations involving 2,4-D or TDZ promoted callus formation but failed to induce shoot differentiation. BA-induced adventitious buds from leaf explants, multiple buds growing from resembling somatic embryos. Meanwhile, BA-induced tissues contained potential meristematic centers, unlike TDZ-induced callus. Higher light intensity (1700–2000&#xa0;lux) drastically improved shoot induction over low light. For rooting, the highest and the healthiest rooting were achieved on NAA-free medium. Regenerated plantlets were successfully acclimatized with a 95% survival rate. These optimized protocols provide a foundation for future genetic improvement of <i>M. normale</i>.</p>

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A regeneration system for Melastoma normale derived from leaf explants: a potential pathway for molecular breeding

  • 期刊论文

  • 发表日期：

    2026年7月3日

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  • Hong Chen,
  • Beiyi Guo,
  • Sixian Zeng,
  • Xinggu Lin,
  • Peishan Zou,
  • Shiou Yih Lee,
  • Guofeng Liu,
  • Seping Dai

摘要

Melastoma normale D. Don is a multifunctional shrub in the genus Melastoma and is highly ornamental. This study establishes an efficient in vitro regeneration system for M. normale and identifies key factors influencing adventitious bud induction. Leaf segments demonstrated exclusive regenerative capacity, with an 84.67% shoot induction rate, whereas petiole and stem explants were unsuitable. The orientation of explants significantly affected shoot regeneration efficiency, with the abaxial side facing the medium yielding superior results. Among basal media, 1/2MS supported optimal regeneration and explant vitality. Plant growth regulators (PGR) combinations critically determined the regeneration pathway. Shoot formation was achieved specifically with 2 mg/L BA and 0.2 mg/L NAA. In contrast, combinations involving 2,4-D or TDZ promoted callus formation but failed to induce shoot differentiation. BA-induced adventitious buds from leaf explants, multiple buds growing from resembling somatic embryos. Meanwhile, BA-induced tissues contained potential meristematic centers, unlike TDZ-induced callus. Higher light intensity (1700–2000 lux) drastically improved shoot induction over low light. For rooting, the highest and the healthiest rooting were achieved on NAA-free medium. Regenerated plantlets were successfully acclimatized with a 95% survival rate. These optimized protocols provide a foundation for future genetic improvement of M. normale.