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<p><i>Vanilla planifolia</i> is an important orchid worldwide, valued for its vanillin content as a natural flavoring. The traditional propagation method using cuttings is slow, labor-intensive, and inefficient. This method compromises the growth of the mother plant. In vitro cultivation presents an alternative for the mass production of healthy vanilla plants. To improve this process, the effect of four cytokinins (6-benzylaminopurine, Kinetin, meta-Topolin, and Thidiazuron) at different concentrations (1.5 to 10.5 µM) was evaluated for shoots multiplication from nodal sections. The study was divided into two phases: Phase 1: assessed the effects of cytokinins in a semisolid medium. The results showed that meta-Topolin (mT) at concentrations of 7.5 and 9.0 µM induced the highest shoot formation per explant (4.52). Phase 2: The most effective mT concentrations from phase 1 were evaluated in an Automated Temporary Immersion Reactor (RITA<sup>®</sup>) under different light wavelengths (fluorescent, Red-Blue LED, White LED, Blue LED, and Red LED). The combination of 9.0 µM mT and white LED light produced the highest number of shoots per explant (17.78). Furthermore, inoculating <i>V. planifolia</i> plants with the fungus <i>T. calospora</i> significantly increased their growth in vitro by more than 30% and in the greenhouse by over 10%, in terms of height, leaf number, and root development, compared with the control group. In conclusion, this study proposes a comprehensive, scalable protocol that combines mT, white LED light, and subsequent inoculation with <i>T. calospora</i>. This method could facilitate the large-scale production of vigorous vanilla plants.</p>

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A comprehensive approach to the in vitro propagation of Vanilla planifolia Andrews: evaluation of different cytokinins and symbiosis with a mycorrhizal fungus

  • 期刊论文

  • 发表日期：

    2026年5月20日

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  • Gabriela García-Vázquez,
  • Antonia Gutiérrez-Mora,
  • J. Francisco Castillo-Esparza,
  • Adrián Alejandro Hernández-Sánchez,
  • Carolina Madero-Vega,
  • Guillermo Ángeles,
  • Gloria Carrión,
  • Martín Mata-Rosas

摘要

Vanilla planifolia is an important orchid worldwide, valued for its vanillin content as a natural flavoring. The traditional propagation method using cuttings is slow, labor-intensive, and inefficient. This method compromises the growth of the mother plant. In vitro cultivation presents an alternative for the mass production of healthy vanilla plants. To improve this process, the effect of four cytokinins (6-benzylaminopurine, Kinetin, meta-Topolin, and Thidiazuron) at different concentrations (1.5 to 10.5 µM) was evaluated for shoots multiplication from nodal sections. The study was divided into two phases: Phase 1: assessed the effects of cytokinins in a semisolid medium. The results showed that meta-Topolin (mT) at concentrations of 7.5 and 9.0 µM induced the highest shoot formation per explant (4.52). Phase 2: The most effective mT concentrations from phase 1 were evaluated in an Automated Temporary Immersion Reactor (RITA®) under different light wavelengths (fluorescent, Red-Blue LED, White LED, Blue LED, and Red LED). The combination of 9.0 µM mT and white LED light produced the highest number of shoots per explant (17.78). Furthermore, inoculating V. planifolia plants with the fungus T. calospora significantly increased their growth in vitro by more than 30% and in the greenhouse by over 10%, in terms of height, leaf number, and root development, compared with the control group. In conclusion, this study proposes a comprehensive, scalable protocol that combines mT, white LED light, and subsequent inoculation with T. calospora. This method could facilitate the large-scale production of vigorous vanilla plants.