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<p><i>Chenopodium quinoa</i> (Willd.), a stress-tolerant pseudocereal with exceptional metabolic plasticity, was evaluated as an in vitro platform for tocopherol (vitamin E) production under controlled elicitation. Callus cultures were challenged with abiotic elicitors [Pb(NO₃)₂, NaCl, and PEG-6000] and biotic elicitors [jasmonic acid (JA), salicylic acid (SA), and fungal extracts], and the most promising treatments were subsequently transferred to suspension cultures and a stirred-tank bioreactor system. Moderate Pb stress (100 mM) increased tocopherol content by 49.2% relative to the untreated callus control, whereas JA (100 µM) and Trichoderma viride extract (5% v/v) achieved 64.9% and 74.1% increases, respectively. Scale-up to a 5-L stirred-tank bioreactor operated at 2.5 L initial working volume confirmed transferability of the response; combined 100 mM Pb + 100 µM JA yielded 65.8 ± 3.8 µg/g DW, corresponding to a 31.1% increase over the untreated bioreactor control and a 256% increase over the original untreated callus baseline. These results identify quinoa as a robust experimental platform for stress-induced tocopherol biosynthesis and highlight biotic elicitors as the more sustainable route for future process development.</p>

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Enhanced tocopherol biosynthesis in Chenopodium quinoa through stress-induced elicitation: a novel strategy for secondary metabolite production

  • 期刊论文

  • 发表日期：

    2026年5月22日

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  • Heba Shahin,
  • Eman A. M. Ahmed,
  • Marwa E. Abd El-Sadek

摘要

Chenopodium quinoa (Willd.), a stress-tolerant pseudocereal with exceptional metabolic plasticity, was evaluated as an in vitro platform for tocopherol (vitamin E) production under controlled elicitation. Callus cultures were challenged with abiotic elicitors [Pb(NO₃)₂, NaCl, and PEG-6000] and biotic elicitors [jasmonic acid (JA), salicylic acid (SA), and fungal extracts], and the most promising treatments were subsequently transferred to suspension cultures and a stirred-tank bioreactor system. Moderate Pb stress (100 mM) increased tocopherol content by 49.2% relative to the untreated callus control, whereas JA (100 µM) and Trichoderma viride extract (5% v/v) achieved 64.9% and 74.1% increases, respectively. Scale-up to a 5-L stirred-tank bioreactor operated at 2.5 L initial working volume confirmed transferability of the response; combined 100 mM Pb + 100 µM JA yielded 65.8 ± 3.8 µg/g DW, corresponding to a 31.1% increase over the untreated bioreactor control and a 256% increase over the original untreated callus baseline. These results identify quinoa as a robust experimental platform for stress-induced tocopherol biosynthesis and highlight biotic elicitors as the more sustainable route for future process development.