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<p><i>Bergenia ciliata</i> (Haw.) Sternb. is a medicinal plant used to treat kidney, bladder stones and rheumatoid arthritis. The overexploitation of <i>B. ciliata</i> has subsequently led to a decline in its natural populations; therefore, there is an urgent need to propagate this important medicinal plant. This study developed an efficient and validated in vitro propagation system for <i>B. ciliata</i> using leaf segments. The combination of 4.43 µM BAP (6-benzylaminopurine) and 5.71 µM IAA (indole-3-acetic acid) in Gamborg’s B5 media was the most efficacious, producing multiple shoots (78.58 ± 0.49) with 94.82 ± 0.09% regeneration frequency. After regeneration, 0.46 µM KN (Kinetin) with 1.61 µM NAA (1-naphthaleneacetic acid) in MS (Murashige &amp; Skoog) media was most efficient for leaf expansion and shoot development, whereas ½ MS basal media was most effective for rooting, inducing 11.57 ± 0.33 roots per shoot. Initially, regeneration was observed through scanning electron microscopy, indicating direct shoot bud induction from the explant. Further, the mother and in vitro-regenerated plants were subjected to genetic fidelity, pigment analysis, high-performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC–MS) analysis. The monomorphic banding pattern of ISSR and SCoT markers demonstrated the clonal stability of in vitro regenerated plants. Pigment analysis showed no significant differences between mother and regenerated plants. GC-MS profiling revealed similar compounds, and bergenin assessment through HPLC reported almost identical content within in vitro plants compared to the mother plant.</p>

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Effect of plant growth regulators on direct regeneration in Bergenia ciliata (Haw.) Sternb: molecular and biochemical evaluation for genetic fidelity

  • 期刊论文

  • 发表日期：

    2026年4月28日

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  • Narendra Sharma,
  • Yadunandan Sen,
  • Taskeen Fatima,
  • Suman Singh,
  • Vanila Sharma,
  • Koustubh Diwakar Mashakhetri,
  • Nagaraju Nekkala,
  • Kota Srinivas

摘要

Bergenia ciliata (Haw.) Sternb. is a medicinal plant used to treat kidney, bladder stones and rheumatoid arthritis. The overexploitation of B. ciliata has subsequently led to a decline in its natural populations; therefore, there is an urgent need to propagate this important medicinal plant. This study developed an efficient and validated in vitro propagation system for B. ciliata using leaf segments. The combination of 4.43 µM BAP (6-benzylaminopurine) and 5.71 µM IAA (indole-3-acetic acid) in Gamborg’s B5 media was the most efficacious, producing multiple shoots (78.58 ± 0.49) with 94.82 ± 0.09% regeneration frequency. After regeneration, 0.46 µM KN (Kinetin) with 1.61 µM NAA (1-naphthaleneacetic acid) in MS (Murashige & Skoog) media was most efficient for leaf expansion and shoot development, whereas ½ MS basal media was most effective for rooting, inducing 11.57 ± 0.33 roots per shoot. Initially, regeneration was observed through scanning electron microscopy, indicating direct shoot bud induction from the explant. Further, the mother and in vitro-regenerated plants were subjected to genetic fidelity, pigment analysis, high-performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC–MS) analysis. The monomorphic banding pattern of ISSR and SCoT markers demonstrated the clonal stability of in vitro regenerated plants. Pigment analysis showed no significant differences between mother and regenerated plants. GC-MS profiling revealed similar compounds, and bergenin assessment through HPLC reported almost identical content within in vitro plants compared to the mother plant.