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<p><i>Actinidia kolomikta</i>, one of the most cold-hardy kiwifruit species, is an excellent genetic resource for cold tolerance breeding. Elucidating the molecular mechanisms underlying its cold tolerance is crucial for enhancing cold tolerance in <i>A.kolomikta</i> and related kiwifruit species. WRKY transcription factors are key regulators of plant abiotic stress responses, but their roles in <i>A.kolomikta</i> cold tolerance remain unclear. We found that overexpression of <i>AkWRKY41</i> in transgenic tobacco conferred enhanced cold stability and post-stress recovery capacity compared to wild type (WT) under cold stress. Specifically, <i>AkWRKY41</i> overexpression in transgenic tobacco significantly reduced cytoplasmic membrane damage, increased antioxidant enzyme activities, and improved reactive oxygen species (ROS) scavenging capacity under cold stress. In contrast, <i>AkWRKY41</i>-silenced lines in <i>A.kolomikta</i> showed more severe wilting and significantly decreased cold tolerance relative to WT. These silenced lines also had significantly higher malondialdehyde (MDA) content and relative electrolyte leakage (indicators of membrane damage) than WT plants. Additionally, the expression levels of CBF pathway-related genes (<i>NtCBF1</i>, <i>NtCBF2</i>, <i>NtCBF3</i>, <i>NtICE1</i>, and <i>NtCOR47</i>) were significantly upregulated and enhancing antioxidant defense capacity. This study clarifies the functional role of <i>AkWRKY41</i> in cold stress response and provides a theoretical basis for exploring the cold tolerance regulatory network of <i>A.kolomikta</i> and accelerating cold-resistant kiwifruit breeding.</p>

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A WRKY transcription factor enhances cold tolerance through modulation of the CBF pathway and antioxidant defense mechanisms in kiwifruit

  • 期刊论文

  • 发表日期：

    2026年4月22日

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  • Yingxin Wang,
  • Shipeng Dong,
  • He Zhao,
  • Dan Liu,
  • Ranhong Li

摘要

Actinidia kolomikta, one of the most cold-hardy kiwifruit species, is an excellent genetic resource for cold tolerance breeding. Elucidating the molecular mechanisms underlying its cold tolerance is crucial for enhancing cold tolerance in A.kolomikta and related kiwifruit species. WRKY transcription factors are key regulators of plant abiotic stress responses, but their roles in A.kolomikta cold tolerance remain unclear. We found that overexpression of AkWRKY41 in transgenic tobacco conferred enhanced cold stability and post-stress recovery capacity compared to wild type (WT) under cold stress. Specifically, AkWRKY41 overexpression in transgenic tobacco significantly reduced cytoplasmic membrane damage, increased antioxidant enzyme activities, and improved reactive oxygen species (ROS) scavenging capacity under cold stress. In contrast, AkWRKY41-silenced lines in A.kolomikta showed more severe wilting and significantly decreased cold tolerance relative to WT. These silenced lines also had significantly higher malondialdehyde (MDA) content and relative electrolyte leakage (indicators of membrane damage) than WT plants. Additionally, the expression levels of CBF pathway-related genes (NtCBF1, NtCBF2, NtCBF3, NtICE1, and NtCOR47) were significantly upregulated and enhancing antioxidant defense capacity. This study clarifies the functional role of AkWRKY41 in cold stress response and provides a theoretical basis for exploring the cold tolerance regulatory network of A.kolomikta and accelerating cold-resistant kiwifruit breeding.