Scholarlink

<p>Glucosinolates (GSLs) are important functional nutrients in <i>Brassica</i> vegetables and play a key role in plant defense responses. Here, we investigated the function of the R2R3-MYB transcription factor gene <i>BoMYB51</i> in indole glucosinolate biosynthesis in kale. A 1002&#xa0;bp coding sequence of <i>BoMYB51</i> was cloned from kale. Sequence alignment and phylogenetic analysis confirmed that BoMYB51 shares high homology with MYB51 orthologs in other Brassicaceae species and contains conserved R2 and R3 domains. Tissue-specific expression analysis revealed that <i>BoMYB51</i> is predominantly expressed in roots, followed by leaves, with lower expression in siliques and stems. Subcellular localization showed that BoMYB51 is located in the nucleus. Through <i>Agrobacterium</i>-mediated transformation, three independent <i>BoMYB51</i> overexpression lines were generated, showing 13.16- to 34.04-fold higher transcript levels than wild-type plants. In these transgenic lines, the expression of key core structure formation genes in glucosinolate biosynthesis - <i>CYP79B2</i>, <i>CYP79B3</i>, and <i>CYP83B1</i> was significantly upregulated, whereas genes involved in side-chain modification remained largely unchanged. Accordingly, total indole GSL content in the overexpressed lines increased by 4.46-, 5.25-, and 4.34-fold compared with wild-type plants, with glucobrassicin being the predominant component. These findings indicate that <i>BoMYB51</i> plays an important role in regulating indole GSLs in kale by activating core structure biosynthetic genes, providing valuable insights into the transcriptional regulation of indole GSL metabolism in Brassicaceae crops.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Overexpression of BoMYB51 enhances indole glucosinolate accumulation in kale by upregulation core structure formation genes

  • 期刊论文

  • 发表日期：

    2026年4月17日

查看全文
  • Yushu Wang,
  • Yafang Luo,
  • Zezheng Song,
  • Shuai Yan,
  • Tiantian Zhou,
  • Meihui Li,
  • Guohua Dong,
  • Huan Wang

摘要

Glucosinolates (GSLs) are important functional nutrients in Brassica vegetables and play a key role in plant defense responses. Here, we investigated the function of the R2R3-MYB transcription factor gene BoMYB51 in indole glucosinolate biosynthesis in kale. A 1002 bp coding sequence of BoMYB51 was cloned from kale. Sequence alignment and phylogenetic analysis confirmed that BoMYB51 shares high homology with MYB51 orthologs in other Brassicaceae species and contains conserved R2 and R3 domains. Tissue-specific expression analysis revealed that BoMYB51 is predominantly expressed in roots, followed by leaves, with lower expression in siliques and stems. Subcellular localization showed that BoMYB51 is located in the nucleus. Through Agrobacterium-mediated transformation, three independent BoMYB51 overexpression lines were generated, showing 13.16- to 34.04-fold higher transcript levels than wild-type plants. In these transgenic lines, the expression of key core structure formation genes in glucosinolate biosynthesis - CYP79B2, CYP79B3, and CYP83B1 was significantly upregulated, whereas genes involved in side-chain modification remained largely unchanged. Accordingly, total indole GSL content in the overexpressed lines increased by 4.46-, 5.25-, and 4.34-fold compared with wild-type plants, with glucobrassicin being the predominant component. These findings indicate that BoMYB51 plays an important role in regulating indole GSLs in kale by activating core structure biosynthetic genes, providing valuable insights into the transcriptional regulation of indole GSL metabolism in Brassicaceae crops.