Scholarlink

<p><i>Pinus tabuliformis</i> Carr. is an ecologically and economically important conifer whose genetic improvement is limited by a prolonged juvenile phase. In this study, immature zygotic embryos were used as explants to investigate factors affecting embryogenic callus induction and somatic embryogenesis, with the aim of developing a regeneration protocol. Differences were observed during both induction and subsequent embryogenic development. During the induction phase, variation was associated with the maternal genotypes of open-pollinated mother trees, with line C4 showing the highest embryogenic callus induction frequency (2.5%) on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (6-BA). During the subsequent developmental phase, embryogenic cell lines differed markedly in their capacity for somatic embryo formation and maturation, with line 174-2 displaying the highest embryogenic potential. Plant growth regulator (PGR) combinations during the proliferation stage influenced subsequent embryogenic competence, with reduced levels of 2,4-D and 6-BA resulting in decreased somatic embryo production and delayed maturation. Under optimized maturation conditions, normal somatic embryos were obtained and successfully germinated into plantlets, which survived acclimatization and transfer to greenhouse conditions. These results provide a basis for further development of somatic embryogenesis-based propagation in <i>P. tabuliformis</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of a somatic embryogenesis protocol for Pinus tabuliformis

  • 期刊论文

  • 发表日期：

    2026年4月18日

查看全文
  • Xiaoqin Zhang,
  • Yiping Ju,
  • Qianyi Zhang,
  • Haiyao Ma,
  • Mingjia Zhang,
  • Qian Xu,
  • Wenhao Ma,
  • Siyuan Chen,
  • Yingming Fan,
  • Jian Zhao,
  • Jinfeng Zhang

摘要

Pinus tabuliformis Carr. is an ecologically and economically important conifer whose genetic improvement is limited by a prolonged juvenile phase. In this study, immature zygotic embryos were used as explants to investigate factors affecting embryogenic callus induction and somatic embryogenesis, with the aim of developing a regeneration protocol. Differences were observed during both induction and subsequent embryogenic development. During the induction phase, variation was associated with the maternal genotypes of open-pollinated mother trees, with line C4 showing the highest embryogenic callus induction frequency (2.5%) on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (6-BA). During the subsequent developmental phase, embryogenic cell lines differed markedly in their capacity for somatic embryo formation and maturation, with line 174-2 displaying the highest embryogenic potential. Plant growth regulator (PGR) combinations during the proliferation stage influenced subsequent embryogenic competence, with reduced levels of 2,4-D and 6-BA resulting in decreased somatic embryo production and delayed maturation. Under optimized maturation conditions, normal somatic embryos were obtained and successfully germinated into plantlets, which survived acclimatization and transfer to greenhouse conditions. These results provide a basis for further development of somatic embryogenesis-based propagation in P. tabuliformis.