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<p><i>Iphigenia magnifica</i> Ansari and R. S. Rao (Colchicaceae) is a seasonal medicinal herb, endemic to India, distributed on plateaus and open grasslands with well-drained soil on gentle slopes. This plant possesses remarkable therapeutic potential and has been used for antimicrobial and antiinflammation. Currently, this species is under the vulnerable category of IUCN red list, but the natural populations of this species has declined sharply due to unregulated harvesting for traditional medicinal use. Seed dormancy and low germination rates limit natural regeneration. For the first time, the effectiveness of in vivo pre-sowing and in vitro treatment with different growth regulators were evaluated to enhance seed germination and induce healthy corm development. H<sub>2</sub>SO<sub>4</sub> scarified seeds for 20&#xa0;s. significantly enhanced seed germination with 66.7 ± 2.7%, 8.53 ± 0.32 days as a germination speed, 262.98 ± 15.78 of vigor index (VI), and 8.33 ± 0.33 of emergence index (EI) than non-scarified seeds. In this study, we also confirmed that scarified seeds treated with sulfuric acid for 20&#xa0;s. and cultured on half-strength Murashige and Skoog (MS) medium containing 1.5 µM GA<sub>3</sub> proved superior for breaking residual dormancy and enhancing germination (99 ± 0.58%) and healthy plantlets were obtained; hence, it can be used for the propagation of <i>I. magnifica</i>. Corms cultured on MS medium containing 2.5 µM GA<sub>3</sub> showed maximum sprouting (98.67 ± 0.33%). Plantlets derived from seeds and corms were successfully acclimatized under natural conditions, confirming a stable protocol for the multiplication of the endemic seasonal medicinal plant <i>I. magnifica</i>. This protocol can also be used for conservation and sustainable propagation.</p> Graphical Abstract <p></p>

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In-vitro and in-vivo assessment of seed viability, germination and corm induction in the seasonal ethnomedicinally important Iphigenia magnifica

  • 期刊论文

  • 发表日期：

    2026年4月3日

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  • Pravin Sangale,
  • Vikas Naikawadi,
  • Shailendra Kamble,
  • Nikhil Magar,
  • Rahul Zanan

摘要

Iphigenia magnifica Ansari and R. S. Rao (Colchicaceae) is a seasonal medicinal herb, endemic to India, distributed on plateaus and open grasslands with well-drained soil on gentle slopes. This plant possesses remarkable therapeutic potential and has been used for antimicrobial and antiinflammation. Currently, this species is under the vulnerable category of IUCN red list, but the natural populations of this species has declined sharply due to unregulated harvesting for traditional medicinal use. Seed dormancy and low germination rates limit natural regeneration. For the first time, the effectiveness of in vivo pre-sowing and in vitro treatment with different growth regulators were evaluated to enhance seed germination and induce healthy corm development. H2SO4 scarified seeds for 20 s. significantly enhanced seed germination with 66.7 ± 2.7%, 8.53 ± 0.32 days as a germination speed, 262.98 ± 15.78 of vigor index (VI), and 8.33 ± 0.33 of emergence index (EI) than non-scarified seeds. In this study, we also confirmed that scarified seeds treated with sulfuric acid for 20 s. and cultured on half-strength Murashige and Skoog (MS) medium containing 1.5 µM GA3 proved superior for breaking residual dormancy and enhancing germination (99 ± 0.58%) and healthy plantlets were obtained; hence, it can be used for the propagation of I. magnifica. Corms cultured on MS medium containing 2.5 µM GA3 showed maximum sprouting (98.67 ± 0.33%). Plantlets derived from seeds and corms were successfully acclimatized under natural conditions, confirming a stable protocol for the multiplication of the endemic seasonal medicinal plant I. magnifica. This protocol can also be used for conservation and sustainable propagation.

Graphical Abstract