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<p>Plant tissue culture is an important clonal propagation technique that enhances plantlet production of species with limited survival/rooting capacity in nurseries. This study investigated the effects of gas exchange rates, sucrose concentrations, and light quality on the micropropagation of two hybrid clones of <i>Corymbia torelliana × C. citriodora</i>. Propagule productivity, morphophysiological development and metabolic responses in vitro, as well as ex vitro survival of microcuttings were assessed. Explants were cultured in sealed vessels without gas-permeable membranes [14 µL L⁻¹ s⁻¹ CO₂] or with two membranes [25 µL L⁻¹ s⁻¹ CO₂], using JADS culture medium supplemented with 0, 15, or 30&#xa0;g L⁻¹ sucrose. Based on these results, an optimal gas exchange –sucrose condition was selected, and a subsequent experiment evaluated different light sources: fluorescent light, white LED, red/blue LED (3:1), and blue LED. A gas exchange rate of 14 µL L⁻¹ s⁻¹ combined with 15&#xa0;g L⁻¹ sucrose increased propagation productivity. In contrast, a higher gas exchange rate (25 µL L⁻¹ s⁻¹) enhanced primary metabolites, organic acids, amino acids, and superoxide dismutase activity, indicating enhanced metabolic and antioxidant responses. The presence of membranes and sucrose promoted greater photosynthetic activity, while ex vitro survival was up to threefold greater when membranes and 15&#xa0;g L⁻¹ sucrose were used. Light quality significantly affected growth: white LED reduced propagation productivity, blue light promoted shoot elongation, and red/blue light increased biomass accumulation, while the ex vitro survival was nine- to fifteen-fold greater when plantlets were micropropagated under fluorescent light compared with white LED.</p>

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Gas exchange, sucrose supply and light quality modulate metabolic and anatomical responses, enhancing productivity in micropropagated Corymbia torelliana × C. citriodora plantlets

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月11日

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  • Marcus Dhilermando Hora-Souza,
  • Elisandra da Silva Sousa,
  • Michelle Maylla Viana de Almeida,
  • Lana Laene Lima Dias,
  • Quezia Pains Dutra,
  • Tatiane Dulcineia Silva,
  • Michelle Araújo Ferreira,
  • Sandy Bastos Martins,
  • Lucas Soares Miguez,
  • Auxiliadora Oliveira Martins,
  • Wagner Luiz Araujo,
  • Aloisio Xavier,
  • Wagner Campos Otoni

摘要

Plant tissue culture is an important clonal propagation technique that enhances plantlet production of species with limited survival/rooting capacity in nurseries. This study investigated the effects of gas exchange rates, sucrose concentrations, and light quality on the micropropagation of two hybrid clones of Corymbia torelliana × C. citriodora. Propagule productivity, morphophysiological development and metabolic responses in vitro, as well as ex vitro survival of microcuttings were assessed. Explants were cultured in sealed vessels without gas-permeable membranes [14 µL L⁻¹ s⁻¹ CO₂] or with two membranes [25 µL L⁻¹ s⁻¹ CO₂], using JADS culture medium supplemented with 0, 15, or 30 g L⁻¹ sucrose. Based on these results, an optimal gas exchange –sucrose condition was selected, and a subsequent experiment evaluated different light sources: fluorescent light, white LED, red/blue LED (3:1), and blue LED. A gas exchange rate of 14 µL L⁻¹ s⁻¹ combined with 15 g L⁻¹ sucrose increased propagation productivity. In contrast, a higher gas exchange rate (25 µL L⁻¹ s⁻¹) enhanced primary metabolites, organic acids, amino acids, and superoxide dismutase activity, indicating enhanced metabolic and antioxidant responses. The presence of membranes and sucrose promoted greater photosynthetic activity, while ex vitro survival was up to threefold greater when membranes and 15 g L⁻¹ sucrose were used. Light quality significantly affected growth: white LED reduced propagation productivity, blue light promoted shoot elongation, and red/blue light increased biomass accumulation, while the ex vitro survival was nine- to fifteen-fold greater when plantlets were micropropagated under fluorescent light compared with white LED.