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<p><i>Microchirita involucrata</i> (family Gesneriaceae) has recently been reassigned to the genus <i>Microchirita</i>; however, its biological properties and potential applications remain unexplored in Thailand. Although the species is valued for its compact growth habit and attractive floral morphology, its phytochemical potential has received little attention. This study established an in vitro culture platform for <i>M. involucrata</i>, identified explants with high secondary metabolite potential, optimized metabolite accumulation through elicitation, and evaluated the biological activities of elicited root cultures. Thin-layer chromatography (TLC) screening revealed that root tissues contained the highest number of antioxidant-active bands; therefore, in vitro–derived root segments were selected for elicitation using methyl jasmonate (MeJA, 50–200 µM), chitosan (CHT, 50–200&#xa0;mg/L), and β-cyclodextrin (CD, 2–8 mM), applied individually and in combination (MeJA + CD and CHT + CD). Root tissues were extracted using 95% ethanol, whereas metabolites secreted into the culture medium were recovered using ethyl acetate extraction. CD alone and combined treatments significantly enhanced antioxidant activity. Root tissue extracts exhibited higher antioxidant capacity, whereas culture medium extracts showed markedly stronger lipoxygenase (LOX) inhibitory and antibacterial activities. Culture media derived from CD and combined elicitor treatments displayed the highest LOX inhibition, with values ranging from 72.04% to 94.35%. Among all treatments, the MeJA + CD–elicited culture medium extract demonstrated the strongest antibacterial activity, with both the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined to be 0.78&#xa0;mg/mL against <i>Staphylococcus aureus</i>. Against <i>Escherichia coli</i>, the corresponding MIC and MBC values were 1.56 and 3.13&#xa0;mg/mL, respectively. Overall, these findings provide new insights into elicitor-induced secondary metabolism in <i>M. involucrata </i>in vitro root cultures, supporting its relevance as a source of biologically active metabolites.</p>

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Elicitor-mediated enhancement of secondary metabolite accumulation and biological activity in Microchirita involucrata in vitro root cultures

  • 期刊论文

  • 发表日期：

    2026年3月30日

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  • Apinun Limmongkon,
  • Wipaporn Chuaymaung,
  • Pathitta Sasiri,
  • Butsakon Nisaipham,
  • Sirianong Khongwet,
  • Onrut Sapatee,
  • Thanakorn Wongsa,
  • Anupan Kongbangkerd,
  • Wannapa Khanthit,
  • Sirinan Temwong,
  • Arpassara Maliprom,
  • Wuthinant Piriyanupong

摘要

Microchirita involucrata (family Gesneriaceae) has recently been reassigned to the genus Microchirita; however, its biological properties and potential applications remain unexplored in Thailand. Although the species is valued for its compact growth habit and attractive floral morphology, its phytochemical potential has received little attention. This study established an in vitro culture platform for M. involucrata, identified explants with high secondary metabolite potential, optimized metabolite accumulation through elicitation, and evaluated the biological activities of elicited root cultures. Thin-layer chromatography (TLC) screening revealed that root tissues contained the highest number of antioxidant-active bands; therefore, in vitro–derived root segments were selected for elicitation using methyl jasmonate (MeJA, 50–200 µM), chitosan (CHT, 50–200 mg/L), and β-cyclodextrin (CD, 2–8 mM), applied individually and in combination (MeJA + CD and CHT + CD). Root tissues were extracted using 95% ethanol, whereas metabolites secreted into the culture medium were recovered using ethyl acetate extraction. CD alone and combined treatments significantly enhanced antioxidant activity. Root tissue extracts exhibited higher antioxidant capacity, whereas culture medium extracts showed markedly stronger lipoxygenase (LOX) inhibitory and antibacterial activities. Culture media derived from CD and combined elicitor treatments displayed the highest LOX inhibition, with values ranging from 72.04% to 94.35%. Among all treatments, the MeJA + CD–elicited culture medium extract demonstrated the strongest antibacterial activity, with both the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined to be 0.78 mg/mL against Staphylococcus aureus. Against Escherichia coli, the corresponding MIC and MBC values were 1.56 and 3.13 mg/mL, respectively. Overall, these findings provide new insights into elicitor-induced secondary metabolism in M. involucrata in vitro root cultures, supporting its relevance as a source of biologically active metabolites.