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<p>The genus <i>Ranunculus</i> L., belonging to the buttercup family (Ranunculaceae), is widespread throughout most of the world, except tropical lowlands and Antarctica. <i>Ranunculus</i> species play an important ecological and commercial role due to their ornamental and medicinal properties. These plants are characterised by remarkable morphological, ecological, and genetic diversity. However, numerous problems related to reproductive biology and phytopathological threats are observed in many members of the genus. Therefore, biotechnological techniques are being increasingly used to propagate several species of <i>Ranunculus.</i> This review provides a comprehensive overview of current protocols for the in vitro propagation of <i>Ranunculus</i>, beginning with the first studies conducted in the 1960s. The selection of explants for the initiation of culture, the media and supplements applied, and approaches to both direct and indirect organogenesis (including adventitious shoot regeneration, axillary shoot proliferation, and callus culture), as well as somatic embryogenesis, rooting, and acclimatisation of regenerated plants are presented. Current research directions, future needs, and prospects are discussed to highlight the growing importance of developing efficient, reproducible, and species-tailored biotechnological strategies that can support conservation programmes, enhance commercial production, and address the biological challenges characteristic of this diverse genus.</p>

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Micropropagation and tissue culture of Ranunculus species: a comprehensive review

  • 期刊论文

  • 发表日期：

    2026年3月28日

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  • Dawid Kocot,
  • Barbara Nowak,
  • Marco Savona,
  • Marina Laura

摘要

The genus Ranunculus L., belonging to the buttercup family (Ranunculaceae), is widespread throughout most of the world, except tropical lowlands and Antarctica. Ranunculus species play an important ecological and commercial role due to their ornamental and medicinal properties. These plants are characterised by remarkable morphological, ecological, and genetic diversity. However, numerous problems related to reproductive biology and phytopathological threats are observed in many members of the genus. Therefore, biotechnological techniques are being increasingly used to propagate several species of Ranunculus. This review provides a comprehensive overview of current protocols for the in vitro propagation of Ranunculus, beginning with the first studies conducted in the 1960s. The selection of explants for the initiation of culture, the media and supplements applied, and approaches to both direct and indirect organogenesis (including adventitious shoot regeneration, axillary shoot proliferation, and callus culture), as well as somatic embryogenesis, rooting, and acclimatisation of regenerated plants are presented. Current research directions, future needs, and prospects are discussed to highlight the growing importance of developing efficient, reproducible, and species-tailored biotechnological strategies that can support conservation programmes, enhance commercial production, and address the biological challenges characteristic of this diverse genus.