Scholarlink

<p><i>Phyllostachys edulis</i> (moso bamboo) holds a significant position as a predominant non-wood forest resource. To advance the breeding of bamboo, we established and optimized a suspension culture system based on embryogenic callus, achieving plant regeneration through somatic embryogenesis. Immature embryos of <i>P. edulis</i> were efficiently induced on Woody Plant Medium (WPM) medium supplemented with 4–6&#xa0;mg/L 2,4-D to form callus, then cultured on WPM medium containing 1&#xa0;mg/L 2,4-D and 0.1&#xa0;mg/L BAP to generate friable embryogenic callus suitable for suspension culture. The suspension culture conditions were optimized for sustained proliferation, with the key parameters set as follows: an initial inoculum volume of 6 mL, pH 5.8, and shaker rotated speed at 85 revolutions per minute (rpm). Embryogenic callus growth followed a typical S-shaped curve over a 28-day culture period, with the exponential growth phase occurring between days 7 and 14. Notably, the addition of ammonium ions (NH₄⁺) significantly promoted embryogenic callus proliferation while reducing callus browning. When transferred callus to liquid WPM medium containing 15% PEG, the nuclei of the callus cells became larger, the cell vitality increased, and embryoids were formed at an efficiency of 24.09%. Moreover, the embryogenic callus developed into somatic embryos and exhibited upregulated expression of embryogenesis-related genes, including <i>PeARF1</i>, <i>PeSERK1</i>, and <i>PePLT5</i>, as well as the shoot regeneration-related gene <i>PeGRF4</i>. This systematic approach provides a robust platform for genetic transformation, germplasm conservation, and the development of synthetic seeds in bamboo species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

An efficient embryogenic cell suspension enabling somatic embryogenesis in Phyllostachys edulis

  • 期刊论文

  • 发表日期：

    2026年4月14日

查看全文
  • Simeng Kong,
  • Huan Liu,
  • Kaiting Wang,
  • Xiaohong Zhou,
  • Xinyi Yang,
  • Juan Zhuo,
  • Xiangyu Li,
  • Xinchun Lin

摘要

Phyllostachys edulis (moso bamboo) holds a significant position as a predominant non-wood forest resource. To advance the breeding of bamboo, we established and optimized a suspension culture system based on embryogenic callus, achieving plant regeneration through somatic embryogenesis. Immature embryos of P. edulis were efficiently induced on Woody Plant Medium (WPM) medium supplemented with 4–6 mg/L 2,4-D to form callus, then cultured on WPM medium containing 1 mg/L 2,4-D and 0.1 mg/L BAP to generate friable embryogenic callus suitable for suspension culture. The suspension culture conditions were optimized for sustained proliferation, with the key parameters set as follows: an initial inoculum volume of 6 mL, pH 5.8, and shaker rotated speed at 85 revolutions per minute (rpm). Embryogenic callus growth followed a typical S-shaped curve over a 28-day culture period, with the exponential growth phase occurring between days 7 and 14. Notably, the addition of ammonium ions (NH₄⁺) significantly promoted embryogenic callus proliferation while reducing callus browning. When transferred callus to liquid WPM medium containing 15% PEG, the nuclei of the callus cells became larger, the cell vitality increased, and embryoids were formed at an efficiency of 24.09%. Moreover, the embryogenic callus developed into somatic embryos and exhibited upregulated expression of embryogenesis-related genes, including PeARF1, PeSERK1, and PePLT5, as well as the shoot regeneration-related gene PeGRF4. This systematic approach provides a robust platform for genetic transformation, germplasm conservation, and the development of synthetic seeds in bamboo species.