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<p><i>Camptotheca acuminata</i>, a distinctive deciduous tree in the family Nyssaceae, belonging to the genus Camptotheca, holds significant economic and medicinal value due to its production of the anticancer compound camptothecin (CPT). In this study, we established an in vitro culture system for cambial meristematic cells (CMCs) in <i>C. acuminata</i> and determined the optimal conditions for their proliferation and subculture through medium optimization. The identity of CMCs was confirmed based on cellular morphology observation and marker gene expression. Furthermore, transcriptomic analysis during CMC proliferation revealed that, using a threshold of |log2(Fold Change)| ≥ 2 (<i>p</i> &lt; 0.05), a total of 3,522 differentially expressed genes (DEGs) were identified during CMC proliferation compared to the initial stage. Among these, 1,647 overlapping DEGs were found, suggesting these genes may play important regulatory roles in CMC proliferation. By further investigating the regulatory pathways and functional genes involved in CMC proliferation, it was found that plant hormone signal transduction was involved in the development of CMCs, with the auxin signaling pathway being particularly active. Auxin signaling integrated with cytokinin and brassinosteroid pathways to jointly regulate the proliferation and state maintenance of CMCs by activating downstream genes that promote cell division. The establishment of the CMC culture system in <i>C. acuminata</i> and the elucidation of the regulatory mechanisms during this process provided a critical foundation for further CMC suspension culture and the induction of CPT production in <i>C. acuminata</i>.</p>

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Acquisition and temporal transcriptomic characteristics of cambial meristematic cells in Camptotheca acuminata under in vitro culture conditions

  • 期刊论文

  • 发表日期：

    2026年3月24日

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  • Yundan Xiong,
  • Shuang Yu,
  • Jiale Sun,
  • Zhiyu Chen,
  • Fanfan Lin,
  • Yifan Cai,
  • Tianping Huang,
  • Xin Yin,
  • Yan Zheng,
  • Yunqiang Yang,
  • Xiangxiang Kong,
  • Yongping Yang

摘要

Camptotheca acuminata, a distinctive deciduous tree in the family Nyssaceae, belonging to the genus Camptotheca, holds significant economic and medicinal value due to its production of the anticancer compound camptothecin (CPT). In this study, we established an in vitro culture system for cambial meristematic cells (CMCs) in C. acuminata and determined the optimal conditions for their proliferation and subculture through medium optimization. The identity of CMCs was confirmed based on cellular morphology observation and marker gene expression. Furthermore, transcriptomic analysis during CMC proliferation revealed that, using a threshold of |log2(Fold Change)| ≥ 2 (p < 0.05), a total of 3,522 differentially expressed genes (DEGs) were identified during CMC proliferation compared to the initial stage. Among these, 1,647 overlapping DEGs were found, suggesting these genes may play important regulatory roles in CMC proliferation. By further investigating the regulatory pathways and functional genes involved in CMC proliferation, it was found that plant hormone signal transduction was involved in the development of CMCs, with the auxin signaling pathway being particularly active. Auxin signaling integrated with cytokinin and brassinosteroid pathways to jointly regulate the proliferation and state maintenance of CMCs by activating downstream genes that promote cell division. The establishment of the CMC culture system in C. acuminata and the elucidation of the regulatory mechanisms during this process provided a critical foundation for further CMC suspension culture and the induction of CPT production in C. acuminata.